Federico Ponzetto1,2, Julien Boccard3,4, Raul Nicoli1, Tiia Kuuranne1, Martial Saugy2, Serge Rudaz3,4. 1. Swiss Laboratory for Doping Analyses, University Center of Legal Medicine Geneva & Lausanne, Centre Hospitalier Universitaire Vaudois & University of Lausanne, 1066 Epalinges, Switzerland. 2. Center of Research & Expertise in Anti-Doping Sciences (REDs), University of Lausanne, 1015 Lausanne, Switzerland. 3. School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneve, Switzerland. 4. Swiss Center of Applied Human Toxicology (SCAHT), University of Basel, 4055 Basel, Switzerland.
Abstract
Aim: Quantification of testosterone (T) and 5α-dihydrotestosterone serum concentrations proved to be an efficient alternative to urinary steroid profiling for the detection of T doping. In this context, additional serum markers could be discovered by exploratory untargeted steroidomics studies. Results: Endogenous steroid metabolites were monitored by ultra high-performance liquid chromatography coupled to high-resolution mass spectrometry in serum samples collected during a T administration clinical trial. A three-step workflow for accurate review of annotation was used and multifactorial data analysis allowed highlighting promising serum biomarkers. Longitudinal monitoring of selected compounds was performed to assess T abuse detection capabilities. Conclusion: Application of serum steroidomics showed high potential for biomarker discovery of T doping, suggesting longitudinal monitoring of steroid hormones in serum as a significant improvement in detection of endogenous steroids abuse.
Aim: Quantification of testosterone (T) and 5α-dihydrotestosterone serum concentrations proved to be an efficient alternative to urinary steroid profiling for the detection of T doping. In this context, additional serum markers could be discovered by exploratory untargeted steroidomics studies. Results: Endogenous steroid metabolites were monitored by ultra high-performance liquid chromatography coupled to high-resolution mass spectrometry in serum samples collected during a T administration clinical trial. A three-step workflow for accurate review of annotation was used and multifactorial data analysis allowed highlighting promising serum biomarkers. Longitudinal monitoring of selected compounds was performed to assess T abuse detection capabilities. Conclusion: Application of serum steroidomics showed high potential for biomarker discovery of T doping, suggesting longitudinal monitoring of steroid hormones in serum as a significant improvement in detection of endogenous steroids abuse.
Entities:
Keywords:
HRMS; UHPLC; doping; multifactorial data analysis; serum; steroidomics; testosterone