Xiaoling Yu1, Zhaofang Han2, Chengsong Ye3, Shuyan Zhou1, Shaogui Wu1, Lifen Han1, Ling Zheng1, Yiqun Hu4, Hanhui Ye5. 1. Infectious Diseases Department, Mengchao Hepatobiliary Hospital of Fujian Medical University, 312 Xihong Road, Gulou District, Fuzhou, 350025, Fujian, China. 2. Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, 43 Yindou Road, Xiamen, 361021, Fujian, China. 3. Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, Fujian, China. 4. Department of Gastroenterology, Zhongshan Hospital of Xiamen University, 201 Hubin Road, Xiamen, 361004, Fujian, China. Electronic address: hyq0826@yahoo.com. 5. Infectious Diseases Department, Mengchao Hepatobiliary Hospital of Fujian Medical University, 312 Xihong Road, Gulou District, Fuzhou, 350025, Fujian, China. Electronic address: 15960102808@163.com.
Abstract
OBJECTIVES: Pseudomonas aeruginosa is a common Gram-negative bacterium causing various serious infections, such as lower respiratory tract infection and urinary tract infection in catheterised patients. Here we report the draft genome sequence of a carbapenem-resistant P. aeruginosa (CRPA) isolate. METHODS: The genome of the CRPA isolate was sequenced using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. RESULTS: The resulting assembly was highly contiguous, containing a total of 6624003bp with a GC content of 66.21%. Annotation identified 6389 protein-coding genes. Mutations in the oprD and mexR genes conferred resistance to carbapenems in the CRPA isolate. CONCLUSION: The draft genome sequence of this CRPA isolate could provide a solid basis for further research on the resistance mechanisms and the development of drug therapy for drug resistance genes.
OBJECTIVES:Pseudomonas aeruginosa is a common Gram-negative bacterium causing various serious infections, such as lower respiratory tract infection and urinary tract infection in catheterised patients. Here we report the draft genome sequence of a carbapenem-resistant P. aeruginosa (CRPA) isolate. METHODS: The genome of the CRPA isolate was sequenced using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. RESULTS: The resulting assembly was highly contiguous, containing a total of 6624003bp with a GC content of 66.21%. Annotation identified 6389 protein-coding genes. Mutations in the oprD and mexR genes conferred resistance to carbapenems in the CRPA isolate. CONCLUSION: The draft genome sequence of this CRPA isolate could provide a solid basis for further research on the resistance mechanisms and the development of drug therapy for drug resistance genes.