Nian Liu1, Liying Zhu2, Zhidong Zhang3, He Huang4, Ling Jiang5. 1. College of Food Science and Light Industry, Nanjing Tech University, Nanjing, People's Republic of China. 2. College of Chemical and Molecular Engineering, Nanjing Tech University, Nanjing, People's Republic of China. Electronic address: zlyhappy@njtech.edu.cn. 3. Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang Uigur Autonomous Region, People's Republic of China. 4. College of Pharmaceutical Science, Nanjing Tech University, Nanjing, People's Republic of China. 5. College of Food Science and Light Industry, Nanjing Tech University, Nanjing, People's Republic of China. Electronic address: jiangling@njtech.edu.cn.
Abstract
OBJECTIVES: Acinetobacter baumannii (A. baumannii) is the world's most significant opportunistic pathogen that causes nosocomial infections; there is widespread health concern due to its ability to acquire multidrug resistance. This study reports a new variety of A. baumannii L13 isolated from Tarim River in Xinjiang Uygur Autonomous Region of China, with β-lactamase resistance gene OXA-69 in the genome. METHODS: Genomic DNA was extracted using an E.Z.N.A.® Bacterial DNA Kit according to the manufacturer's recommended protocol. The genome of A. baumannii L13 was sequenced using an Illumina HiSeq™ 2000 sequencing system, assembled with SOAPdenovo and optimised with GapCloser. The rRNAs were predicted by barrnap, and tRNAs were predicted by tRNA-scan-SE. Resistance genes were analysed using CARD databases. RESULTS: The whole genome size of A. baumannii L13 was calculated at 3 969 074 base pairs (bp), which was assembled into 60 contigs and 56 scaffolds (>1000 bp length), with a G + C content of 38.9% and N rate of 0.001%. The number of CDSs was 3815, with length accounting for approximately 85.61% of the whole genome, 7.6% and 8.2% of which were related to virulence genes and multidrug-resistance genes, respectively. CONCLUSIONS: The genome sequence of A. baumannii L13 reported here will benefit comparative analysis of the Acinetobacter genus, and promote further understanding of the specific genomic feature in terms of multidrug resistance in A. baumannii.
OBJECTIVES:Acinetobacter baumannii (A. baumannii) is the world's most significant opportunistic pathogen that causes nosocomial infections; there is widespread health concern due to its ability to acquire multidrug resistance. This study reports a new variety of A. baumannii L13 isolated from Tarim River in Xinjiang Uygur Autonomous Region of China, with β-lactamase resistance gene OXA-69 in the genome. METHODS: Genomic DNA was extracted using an E.Z.N.A.® Bacterial DNA Kit according to the manufacturer's recommended protocol. The genome of A. baumannii L13 was sequenced using an Illumina HiSeq™ 2000 sequencing system, assembled with SOAPdenovo and optimised with GapCloser. The rRNAs were predicted by barrnap, and tRNAs were predicted by tRNA-scan-SE. Resistance genes were analysed using CARD databases. RESULTS: The whole genome size of A. baumannii L13 was calculated at 3 969 074 base pairs (bp), which was assembled into 60 contigs and 56 scaffolds (>1000 bp length), with a G + C content of 38.9% and N rate of 0.001%. The number of CDSs was 3815, with length accounting for approximately 85.61% of the whole genome, 7.6% and 8.2% of which were related to virulence genes and multidrug-resistance genes, respectively. CONCLUSIONS: The genome sequence of A. baumannii L13 reported here will benefit comparative analysis of the Acinetobacter genus, and promote further understanding of the specific genomic feature in terms of multidrug resistance in A. baumannii.