Hoda Derakhshanian1,2, Abolghassem Djazayery3, Mohammad Hassan Javanbakht4, Mohammad Reza Eshraghian5, Abbas Mirshafiey6, Samane Jahanabadi7, Sajad Ghadbeigi8, Mahnaz Zarei4, Ehsan Alvandi4, Mahmoud Djalali4. 1. Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran. 2. Department of Biochemistry and Nutrition, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran. 3. Department of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. 4. Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. 5. Department of Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. 6. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. 7. Department of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 8. Department of Medicinal Chemistry, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Abstract
OBJECTIVE: Vitamin D deficiency has been reported to be associated with the incidence of type 1 and type 2 diabetes and worsening of diabetes complications. This study was designed to investigate the effect of vitamin D treatment on the expression of five key genes involved in the development of diabetic cardiomyopathy. METHODS: Twenty-four male Sprague-Dawley rats were randomly divided into three groups. The first group served as control and the other two groups received an intraperitoneal injection of 45 mg/kg streptozotocin (STZ) to develop diabetes. Then groups were treated for 4 weeks either with placebo or vitamin D (two injections of 20,000 IU/kg). Serum levels of glucose, insulin, HbA1c, and advanced glycation end products (AGEs), as well as the gene expression of AGE cellular receptor (RAGE), glyoxalase, aldose reductase, O-GlcNAc transferase (OGT), and glutamine-fructose-6-phosphate aminotransferase (GFAT) and nuclear factor-kB (NF-kB) activity of nuclear extracts were assessed at the end of experiment. RESULTS: Increment in serum cholecalciferol could improve hyperglycaemia and hypoinsulinemia in diabetic rats. In addition, a significant reduction was observed in RAGE, OGT, and GFAT gene expression and NF-kB activity in cardiac myocytes. CONCLUSIONS: Vitamin D might contribute in reducing diabetic cardiomyopathy not only by improving blood glucose and insulin levels but also via downregulating AGE and hexosamine pathways and decreasing NF-kB activity in heart tissue.
OBJECTIVE:Vitamin D deficiency has been reported to be associated with the incidence of type 1 and type 2 diabetes and worsening of diabetes complications. This study was designed to investigate the effect of vitamin D treatment on the expression of five key genes involved in the development of diabetic cardiomyopathy. METHODS: Twenty-four male Sprague-Dawley rats were randomly divided into three groups. The first group served as control and the other two groups received an intraperitoneal injection of 45 mg/kg streptozotocin (STZ) to develop diabetes. Then groups were treated for 4 weeks either with placebo or vitamin D (two injections of 20,000 IU/kg). Serum levels of glucose, insulin, HbA1c, and advanced glycation end products (AGEs), as well as the gene expression of AGE cellular receptor (RAGE), glyoxalase, aldose reductase, O-GlcNAc transferase (OGT), and glutamine-fructose-6-phosphate aminotransferase (GFAT) and nuclear factor-kB (NF-kB) activity of nuclear extracts were assessed at the end of experiment. RESULTS: Increment in serum cholecalciferol could improve hyperglycaemia and hypoinsulinemia in diabeticrats. In addition, a significant reduction was observed in RAGE, OGT, and GFAT gene expression and NF-kB activity in cardiac myocytes. CONCLUSIONS:Vitamin D might contribute in reducing diabetic cardiomyopathy not only by improving blood glucose and insulin levels but also via downregulating AGE and hexosamine pathways and decreasing NF-kB activity in heart tissue.
Authors: Silvia Savastio; Erica Pozzi; Francesco Tagliaferri; Roberta Degrandi; Roberta Cinquatti; Ivana Rabbone; Gianni Bona Journal: Int J Mol Sci Date: 2020-05-16 Impact factor: 5.923
Authors: Awad S Alsamghan; Safar A Alsaleem; Mohammed A S Alzahrani; Ayyub Patel; Ayaz K Mallick; Salah A Sheweita Journal: Oxid Med Cell Longev Date: 2020-12-23 Impact factor: 6.543