L Li1, R Zhang, S-J Li. 1. Department of Gynecology, Weifang People's Hospital, Weifang, China. 977980981@qq.com.
Abstract
OBJECTIVE: The ovarian cancer is one of the most common fatal cancers. Recently, the role of long noncoding RNAs (lncRNAs) in tumor progression has attracted much attention in researchers. The aim of this study was to investigate the role of lncRNA SNHG14 in the progression of ovarian cancer and to explore the possible mechanism. PATIENTS AND METHODS: Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was utilized to detect SNHG14 expression in ovarian cancer tissues. To identify the function of SNHG14 in ovarian cancer, functional experiments were conducted in vitro and in vivo. In addition, the luciferase assays and RNA immunoprecipitation assay (RIP) were performed to investigate the underlying mechanism. RESULTS: SNHG14 expression was remarkably higher in ovarian cancer tissues than that of corresponding normal tissues. SNHG14 expression was associated with patients' overall survival time as well. After SNHG14 was silenced in ovarian cancer cells, cell proliferation, migration, and invasion were remarkably inhibited. In addition, the expression of miR-219a-5p was significantly up-regulated after the silence of SNHG14. Further mechanism assays showed that miR-219a-5p was a direct target of SNHG14 in ovarian cancer. CONCLUSIONS: SNHG14 serves as a potential oncogene in ovarian cancer. In addition, it enhances ovarian cell metastasis and proliferation via sponging miR-219a-5p.
OBJECTIVE: The ovarian cancer is one of the most common fatal cancers. Recently, the role of long noncoding RNAs (lncRNAs) in tumor progression has attracted much attention in researchers. The aim of this study was to investigate the role of lncRNA SNHG14 in the progression of ovarian cancer and to explore the possible mechanism. PATIENTS AND METHODS: Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was utilized to detect SNHG14 expression in ovarian cancer tissues. To identify the function of SNHG14 in ovarian cancer, functional experiments were conducted in vitro and in vivo. In addition, the luciferase assays and RNA immunoprecipitation assay (RIP) were performed to investigate the underlying mechanism. RESULTS:SNHG14 expression was remarkably higher in ovarian cancer tissues than that of corresponding normal tissues. SNHG14 expression was associated with patients' overall survival time as well. After SNHG14 was silenced in ovarian cancer cells, cell proliferation, migration, and invasion were remarkably inhibited. In addition, the expression of miR-219a-5p was significantly up-regulated after the silence of SNHG14. Further mechanism assays showed that miR-219a-5p was a direct target of SNHG14 in ovarian cancer. CONCLUSIONS:SNHG14 serves as a potential oncogene in ovarian cancer. In addition, it enhances ovarian cell metastasis and proliferation via sponging miR-219a-5p.