| Literature DB >> 31172476 |
Jacek Zielonka1,2,3, Monika Zielonka4,5, Balaraman Kalyanaraman4,5,6.
Abstract
Hydroethidine is a fluorogenic probe that in the presence of the superoxide radical anion is oxidized to a red fluorescent product, 2-hydroxyethidium. In cells, hydroethidine is also oxidized to other products, including red fluorescent ethidium. Thus, selective monitoring of 2-hydroxyethidium is required for specific detection of the superoxide radical anion. Here, we provide protocols for HPLC- and LC-MS-based quantitation of 2-hydroxyethidium, among other oxidation products. Also, a protocol for continuous sampling for real-time monitoring of superoxide production using rapid HPLC measurements of 2-hydroxyethidium is described.Entities:
Keywords: 2-hydroxyethidium; HPLC; Hydroethidine; LC-MS; Superoxide radical anion
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Year: 2019 PMID： 31172476 PMCID： PMC6953631 DOI： 10.1007/978-1-4939-9424-3_14
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745