Analysis of four parabens and bisphenols A, F, S in urine, using dilute and shoot and liquid chromatography coupled to mass spectrometry.

A new strategy for the analysis of 4 parabens (methylparaben, ethyl paraben, propyl paraben and butyl paraben) and 3 bisphenols (A, F and S) in human urine has been developed. Dilute and shoot and liquid chromatography coupled to mass spectrometry (LC-MS/MS) determination was employed. Procedural matched calibration, certified materials and spiked samples were used for validation. The obtained recoveries varied between 85 and 104% with a precision lower than 20% for all analytes. The LOQ was 0.20 ng mL for all analytes. Negative atmospheric pressure chemical ionization in the selected reaction monitoring mode was used for MS detection. The proposed method was successfully applied for the determination of these compounds in 15 human urine samples from lactating women from the Valencian region (Spain). Methyl paraben (MP) and Bisphenol A (BPA) presented high detection frequency (93% for MP and 80% for BPA), BPS and BPF presented a detection frequency of 33 and 26% respectively. Methyl paraben was detected in a concentration range of 0.2-2952 ng mL-1, 0.2-12 ng mL-1 for bisphenol A, 0.5-39 ng mL-1 for BPF and 0.5-8.5 ng mL-1 for BPS.