Literature DB >> 3117054

Primary structure around the lipoate-attachment site on the E2 component of bovine heart pyruvate dehydrogenase complex.

A P Bradford1, S Howell, A Aitken, L A James, S J Yeaman.   

Abstract

Bovine heart pyruvate dehydrogenase complex was acetylated by using [3-14C]pyruvate in the presence of N-ethylmaleimide, with approx. 1 mol of acetyl groups being incorporated per mol of E2 polypeptide. After peptic digestion, lipoate-containing peptides were purified by high-voltage electrophoresis and ion-exchange and reverse-phase h.p.l.c. The amino acid sequence around the lipoic acid-attachment site of E2 was determined by automated Edman degradation. Acetylation of a lipoate cofactor bound to a lysine residue was verified by fast-atom-bombardment m.s.

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Year:  1987        PMID： 3117054      PMCID： PMC1148219          DOI： 10.1042/bj2450919

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  18 in total

1.  Acyl group and electron pair relay system: a network of interacting lipoyl moieties in the pyruvate and alpha-ketoglutarate dehydrogenase complexes from Escherichia coli.

Authors:  J H Collins; L J Reed
Journal:  Proc Natl Acad Sci U S A       Date:  1977-10       Impact factor: 11.205

2.  Amidination of pyruvate dehydrogenase complex of Escherichia coli under denaturing conditions.

Authors:  G Hale; E A Hooper; R N Perham
Journal:  Biochem J       Date:  1979-01-01       Impact factor: 3.857

3.  -Keto acid dehydrogenase complexes. XV. Purification and properties of the component enzymes of the pyruvate dehydrogenase complexes from bovine kidney and heart.

Authors:  T C Linn; J W Pelley; F H Pettit; F Hucho; D D Randall; L J Reed
Journal:  Arch Biochem Biophys       Date:  1972-02       Impact factor: 4.013

4.  Purification of 2-oxo acid dehydrogenase multienzyme complexes from ox heart by a new method.

Authors:  C J Stanley; R N Perham
Journal:  Biochem J       Date:  1980-10-01       Impact factor: 3.857

5.  Function and regulation of mammalian pyruvate dehydrogenase complex. Acetylation, interlipoyl acetyl transfer, and migration of the pyruvate dehydrogenase component.

Authors:  R L Cate; T E Roche
Journal:  J Biol Chem       Date:  1979-03-10       Impact factor: 5.157

6.  Subunit structure of dihydrolipoyl transacetylase component of pyruvate dehydrogenase complex from bovine heart.

Authors:  D M Bleile; M L Hackert; F H Pettit; L J Reed
Journal:  J Biol Chem       Date:  1981-01-10       Impact factor: 5.157

7.  Lipoic acid content of dihydrolipoyl transacylases determined by isotope dilution analysis.

Authors:  R H White; D M Bleile; L J Reed
Journal:  Biochem Biophys Res Commun       Date:  1980-05-14       Impact factor: 3.575

8.  Conformational mobility of polypeptide chains in the 2-oxo acid dehydrogenase complexes from ox heart revealed by proton NMR spectroscopy.

Authors:  E J Wawrzynczak; R N Perham; G C Roberts
Journal:  FEBS Lett       Date:  1981-08-17       Impact factor: 4.124

9.  Dihydrolipoyl transacetylase of Escherichia coli. Formation of 8-S-acetyldihydrolipoamide.

Authors:  Y S Yang; P A Frey
Journal:  Biochemistry       Date:  1986-12-16       Impact factor: 3.162

10.  13C nuclear magnetic resonance study of the pyruvate dehydrogenase-catalyzed acetylation of dihydrolipoamide.

Authors:  T P O'Connor; T E Roche; J V Paukstelis
Journal:  J Biol Chem       Date:  1982-03-25       Impact factor: 5.157
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  7 in total

Review 1.  The 2-oxo acid dehydrogenase complexes: recent advances.

Authors:  S J Yeaman
Journal:  Biochem J       Date:  1989-02-01       Impact factor: 3.857

Review 2.  Primary biliary cirrhosis: nature of autoantigens.

Authors:  M F Bassendine; S J Yeaman
Journal:  Springer Semin Immunopathol       Date:  1990

3.  T cell responses to the putative dominant autoepitope in primary biliary cirrhosis (PBC).

Authors:  J M Palmer; A G Diamond; S J Yeaman; M F Bassendine; D E Jones
Journal:  Clin Exp Immunol       Date:  1999-04       Impact factor: 4.330

4.  Identification and analysis of the major M2 autoantigens in primary biliary cirrhosis.

Authors:  S P Fussey; J R Guest; O F James; M F Bassendine; S J Yeaman
Journal:  Proc Natl Acad Sci U S A       Date:  1988-11       Impact factor: 11.205

5.  Primary structure of the human M2 mitochondrial autoantigen of primary biliary cirrhosis: dihydrolipoamide acetyltransferase.

Authors:  R L Coppel; L J McNeilage; C D Surh; J Van de Water; T W Spithill; S Whittingham; M E Gershwin
Journal:  Proc Natl Acad Sci U S A       Date:  1988-10       Impact factor: 11.205

6.  Expression and lipoylation in Escherichia coli of the inner lipoyl domain of the E2 component of the human pyruvate dehydrogenase complex.

Authors:  J Quinn; A G Diamond; A K Masters; D E Brookfield; N G Wallis; S J Yeaman
Journal:  Biochem J       Date:  1993-01-01       Impact factor: 3.857

7.  The autoepitope of the 74-kD mitochondrial autoantigen of primary biliary cirrhosis corresponds to the functional site of dihydrolipoamide acetyltransferase.

Authors:  J Van de Water; M E Gershwin; P Leung; A Ansari; R L Coppel
Journal:  J Exp Med       Date:  1988-06-01       Impact factor: 14.307
  7 in total

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