Hiroki Kajita1, Anna Oh1, Moemi Urano2, Masashi Takemaru1, Nobuaki Imanishi2, Marika Otaki1, Takayuki Yagi2,3, Sadakazu Aiso2,4,5, Kazuo Kishi1. 1. Department of Plastic and Reconstructive Surgery, Keio University School of Medicine, Tokyo, Japan. 2. Department of Anatomy, Keio University School of Medicine, Tokyo, Japan. 3. Department of Technology Development, Luxonus Inc., Kawasaki, Kanagawa, Japan. 4. SIT Research Laboratories, Shibaura Institute of Technology, Tokyo, Japan. 5. Management Division, Luxonus Inc., Kawasaki, Kanagawa, Japan.
Abstract
BACKGROUND AND OBJECTIVES: Photoacoustic lymphangiography, which is based on photoacoustic technology, is an optical imaging that visualizes the distribution of light absorbing tissue components like hemoglobin or melanin, as well as optical absorption contrast imaging agents like indocyanine green (ICG) in the lymphatic channels, with high spatial resolution. In this report, we introduce the three-dimensional (3D) images of human lymphatic vessels obtained with photoacoustic lymphangiography. METHODS: We used the 3D photoacoustic visualization system (PAI-05). Some healthy subjects and lymphedema patients were recruited. To image the lymphatic structures of the limbs ICG was administered subcutaneously as in fluorescence lymphangiography. Photoacoustic images were acquired by irradiating the tissue using a laser at wavelengths of near-infrared region. On the same occasion, fluorescence images were also recorded. RESULTS: The lymphatic vessels up to the diameter of 0.2 mm could be observed three-dimensionally with the venules around them. In the patient-group, dermal backflow patterns were often observed as dense interconnecting 3D structures of lymphatic vessels. Collecting vessels passing below the dermis were also observed, which were not observed by fluorescence lymphography. CONCLUSIONS: Photoacoustic lymphangiography provided the detailed observation of each lymphatic vessel, leading to deeper understanding of 3D structures and physiological state of the vessel.
BACKGROUND AND OBJECTIVES: Photoacoustic lymphangiography, which is based on photoacoustic technology, is an optical imaging that visualizes the distribution of light absorbing tissue components like hemoglobin or melanin, as well as optical absorption contrast imaging agents like indocyanine green (ICG) in the lymphatic channels, with high spatial resolution. In this report, we introduce the three-dimensional (3D) images of human lymphatic vessels obtained with photoacoustic lymphangiography. METHODS: We used the 3D photoacoustic visualization system (PAI-05). Some healthy subjects and lymphedemapatients were recruited. To image the lymphatic structures of the limbs ICG was administered subcutaneously as in fluorescence lymphangiography. Photoacoustic images were acquired by irradiating the tissue using a laser at wavelengths of near-infrared region. On the same occasion, fluorescence images were also recorded. RESULTS: The lymphatic vessels up to the diameter of 0.2 mm could be observed three-dimensionally with the venules around them. In the patient-group, dermal backflow patterns were often observed as dense interconnecting 3D structures of lymphatic vessels. Collecting vessels passing below the dermis were also observed, which were not observed by fluorescence lymphography. CONCLUSIONS: Photoacoustic lymphangiography provided the detailed observation of each lymphatic vessel, leading to deeper understanding of 3D structures and physiological state of the vessel.
Authors: Erin Newcomer; Guang Yang; Bei Sun; Hongbo Luo; Duanwen Shen; Samuel Achilefu; Valerie Ratts; Joan Riley; John Yeh; Quing Zhu Journal: F S Sci Date: 2020-07-14
Authors: Saskia van Heumen; Jonas J M Riksen; Wichor M Bramer; Gijs van Soest; Dalibor Vasilic Journal: Ann Surg Oncol Date: 2022-09-28 Impact factor: 4.339