Ahmed M Abu El-Asrar1,2, Nele Berghmans3, Saleh A Al-Obeidan1, Priscilla W Gikandi1, Ghislain Opdenakker3, Jo Van Damme3, Sofie Struyf3. 1. Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia. 2. Dr. Nasser Al-Rashid Research Chair in Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia. 3. Laboratory of Immunobiology, Rega Institute for Medical Research and Department of Microbiology and Immunology, University of Leuven, KU Leuven, Leuven, Belgium.
Abstract
Purpose: To evaluate expression of cytokines GM-CSF, IL-11, IL-12p40, IL-12p70, IL-27p28, IL-35, APRIL, BAFF, TWEAK, and LIGHT in uveitis. Methods: Aqueous humor samples from patients with active uveitis associated with Behçet's disease (BD), sarcoidosis, HLA-B27-related inflammation, and Vogt-Koyanagi-Harada (VKH) disease and control patients were assayed with a multiplex assay. Results: Comparing all patients to controls, GM-CSF, IL-11, IL-12p40, APRIL, and BAFF were significantly increased, whereas LIGHT was significantly decreased. IL-11 and BAFF were the most strongly upregulated, being elevated 19.7-fold and 14.1-fold, respectively, compared with controls. IL-11 was significantly highest in HLA-B27 uveitis. GM-CSF, IL-11, and IL-12p40 were significantly higher in nongranulomatous uveitis (BD and HLA-B27) than in granulomatous uveitis (sarcoidosis and VKH), whereas APRIL and TWEAK were significantly higher in granulomatous uveitis.Conclusions: IL-11-driven immune responses might be more potent in nongranulomatous uveitis, particularly in HLA-B27 uveitis. BAFF and APRIL might contribute to B cell-driven autoimmune response in uveitis.
Purpose: To evaluate expression of cytokines GM-CSF, IL-11, IL-12p40, IL-12p70, IL-27p28, IL-35, APRIL, BAFF, TWEAK, and LIGHT in uveitis. Methods: Aqueous humor samples from patients with active uveitis associated with Behçet's disease (BD), sarcoidosis, HLA-B27-related inflammation, and Vogt-Koyanagi-Harada (VKH) disease and control patients were assayed with a multiplex assay. Results: Comparing all patients to controls, GM-CSF, IL-11, IL-12p40, APRIL, and BAFF were significantly increased, whereas LIGHT was significantly decreased. IL-11 and BAFF were the most strongly upregulated, being elevated 19.7-fold and 14.1-fold, respectively, compared with controls. IL-11 was significantly highest in HLA-B27uveitis. GM-CSF, IL-11, and IL-12p40 were significantly higher in nongranulomatous uveitis (BD and HLA-B27) than in granulomatous uveitis (sarcoidosis and VKH), whereas APRIL and TWEAK were significantly higher in granulomatous uveitis.Conclusions: IL-11-driven immune responses might be more potent in nongranulomatous uveitis, particularly in HLA-B27uveitis. BAFF and APRIL might contribute to B cell-driven autoimmune response in uveitis.
Authors: L De Simone; M Bonacini; S Croci; L Cimino; R Aldigeri; F Alessandrello; V Mastrofilippo; F Gozzi; E Bolletta; C Adani; A Zerbini; G M Cavallini; L Fontana; C Salvarani Journal: Inflamm Res Date: 2022-06-28 Impact factor: 6.986
Authors: Martina Bonacini; Alessandra Soriano; Luca Cimino; Luca De Simone; Elena Bolletta; Fabrizio Gozzi; Francesco Muratore; Maria Nicastro; Lucia Belloni; Alessandro Zerbini; Luigi Fontana; Carlo Salvarani; Stefania Croci Journal: Front Immunol Date: 2020-03-10 Impact factor: 7.561