| Literature DB >> 31161411 |
Guohua Li1,2, Hongli Jin2,3, Xin Nie4, Yongkun Zhao2,5, Na Feng2,5, Zongxi Cao6, Shuyi Tan6, Bo Zhang7, Weiwei Gai3, Feihu Yan2, Ling Li3,8, Ying Zhang2,9, Zengguo Cao2,3, Nan Li2, Yuwei Gao2,5, Songtao Yang2,5, Xianzhu Xia10,11,12, Hualei Wang13,14.
Abstract
Japanese encephalitis virus SA14-14-2 (JEV SA14-14-2) is a widely used vaccine in China and other southeastern countries to prevent Japanese encephalitis in children. In this study, a stable infectious cDNA clone of JEV SA14-14-2 with a low copy number pACYC177 vector dependent on the T7 promoter and T7 terminator was developed. Two introns were inserted into the capsid gene and envelope gene of JEV cDNA for gene stability. Hepatitis delta virus ribozyme (HDVr) was engineered into the 3' UTR cDNA of JEV for authentic 3' UTR transcription. The rescued virus showed biological properties indistinguishable from those of the parent strain (JEV SA14-14-2). The establishment of a JEV SA14-14-2 reverse genetics system lays the foundation for the further development of other flavivirus vaccines and viral pathogenesis studies.Entities:
Keywords: Infectious clone; Intron; Japanese encephalitis virus; T7 terminator
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Year: 2019 PMID： 31161411 DOI： 10.1007/s11262-019-01674-y
Source DB: PubMed Journal: Virus Genes ISSN： 0920-8569 Impact factor: 2.332