Literature DB >> 3116075

Effect of tissue fixation on anti-bromodeoxyuridine immunohistochemistry.

B Schutte1, M M Reynders, F T Bosman, G H Blijham.   

Abstract

We investigated the influence of various fixatives on monoclonal anti-BrdUrd antibody binding of BrdUrd-substituted DNA in tissue sections of routinely processed mouse small intestine after in vivo administration of BrdUrd. For denaturing fixatives such as ethanol or Carnoy's fluid, a standard denaturation protocol showed specific crypt cell labeling. With cross-linking agents such as formalin and glutaraldehyde, a remarkable increase in staining intensity was obtained after tissue digestion with pepsin before acid denaturation. The optimal pepsin concentration was determined for maximal immunoreactivity combined with acceptable morphology.

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Year:  1987        PMID： 3116075     DOI： 10.1177/35.11.3116075

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  33 in total

1.  Bromodeoxyuridine (BrdU) immunocytochemistry by exonuclease III (Exo III) digestion.

Authors:  W N Dinjens; J ten Kate; M H Lenders; E P van der Linden; F T Bosman
Journal:  Histochemistry       Date:  1992-10

2.  Leukemia inhibitory factor induces differentiation of pituitary corticotroph function: an immuno-neuroendocrine phenotypic switch.

Authors:  B Stefana; D W Ray; S Melmed
Journal:  Proc Natl Acad Sci U S A       Date:  1996-10-29       Impact factor: 11.205

3.  Scale morphogenesis during embryonic development in the lizard Anolis lineatopus.

Authors:  L Alibardi
Journal:  J Anat       Date:  1996-06       Impact factor: 2.610

4.  New sites of methylcytosine-rich DNA detected on metaphase chromosomes.

Authors:  A Barbin; C Montpellier; N Kokalj-Vokac; A Gibaud; A Niveleau; B Malfoy; B Dutrillaux; C A Bourgeois
Journal:  Hum Genet       Date:  1994-12       Impact factor: 4.132

5.  PC10 monoclonal antibody to proliferating cell nuclear antigen as probe for cycling cell detection in developing tissues. A combined immunocytochemical and flow cytometric study.

Authors:  A Casasco; M Giordano; M Danova; M Casasco; A Icaro Cornaglia; A Calligaro
Journal:  Histochemistry       Date:  1993-03

6.  CCAAT enhancer- binding protein beta is required for normal hepatocyte proliferation in mice after partial hepatectomy.

Authors:  L E Greenbaum; W Li; D E Cressman; Y Peng; G Ciliberto; V Poli; R Taub
Journal:  J Clin Invest       Date:  1998-09-01       Impact factor: 14.808

Review 7.  Kinetics of adrenal medullary cells.

Authors:  A A Verhofstad
Journal:  J Anat       Date:  1993-10       Impact factor: 2.610

8.  The proliferative kinetics of somatostatin-producing cells in the rat antral mucosa after truncal vagotomy.

Authors:  H Shimoda; S Yokoyama; I Nakayama; T Hadama; Y Uchida
Journal:  Surg Today       Date:  1992       Impact factor: 2.549

9.  Cell proliferation induced in the kidneys and livers of rats and mice by short term exposure to the carcinogen p-dichlorobenzene.

Authors:  T Umemura; K Tokumo; G M Williams
Journal:  Arch Toxicol       Date:  1992       Impact factor: 5.153

10.  Relaxin acts on stromal cells to promote epithelial and stromal proliferation and inhibit apoptosis in the mouse cervix and vagina.

Authors:  LiJuan Yao; Alexander I Agoulnik; Paul S Cooke; Daryl D Meling; O David Sherwood
Journal:  Endocrinology       Date:  2008-01-24       Impact factor: 4.736
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