| Literature DB >> 31160708 |
Yael Nechemia-Arbely1,2, Karen H Miga3, Ofer Shoshani4, Aaron Aslanian5, Moira A McMahon4,6, Ah Young Lee4, Daniele Fachinetti4,7, John R Yates5, Bing Ren4, Don W Cleveland8.
Abstract
Chromatin assembled with the histone H3 variant CENP-A is the heritable epigenetic determinant of human centromere identity. Using genome-wide mapping and reference models for 23 human centromeres, CENP-A binding sites are identified within the megabase-long, repetitive α-satellite DNAs at each centromere. CENP-A is shown in early G1 to be assembled into nucleosomes within each centromere and onto 11,390 transcriptionally active sites on the chromosome arms. DNA replication is demonstrated to remove ectopically loaded, non-centromeric CENP-A. In contrast, tethering of centromeric CENP-A to the sites of DNA replication through the constitutive centromere associated network (CCAN) is shown to enable precise reloading of centromere-bound CENP-A onto the same DNA sequences as in its initial prereplication loading. Thus, DNA replication acts as an error correction mechanism for maintaining centromere identity through its removal of non-centromeric CENP-A coupled with CCAN-mediated retention and precise reloading of centromeric CENP-A.Entities:
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Year: 2019 PMID： 31160708 PMCID： PMC7015266 DOI： 10.1038/s41556-019-0331-4
Source DB: PubMed Journal: Nat Cell Biol ISSN： 1465-7392 Impact factor: 28.824