| Literature DB >> 31160055 |
Anton Wangler1, Christoph Held2, Gabriele Sadowski1.
Abstract
To improve the kinetics of enzyme-catalyzed reactions, cosolvents are commonly added to reaction mixtures. The search for a good cosolvent is still empirical and experimentally based. We discuss a thermodynamic activity-based approach that improves biocatalytic processes by predicting cosolvent influences on Michaelis constants, ultimately reducing time and cost.Keywords: Michaelis constant; activity coefficients; biocatalysis; cosolvent; ePC-SAFT; predictions
Year: 2019 PMID： 31160055 DOI： 10.1016/j.tibtech.2019.04.015
Source DB: PubMed Journal: Trends Biotechnol ISSN： 0167-7799 Impact factor: 19.536