Highly sensitive and straightforward methods for the detection of cyanide using profluorescent glutathionylcobalamin.

The extreme toxicity of cyanide and its continued use in various industries have raised concerns over environmental contamination and, therefore, considerable attention has given to develop facile and sensitive methods of cyanide detection. In this study, we developed highly sensitive and straightforward methods of cyanide detection using eosin-labeled glutathionylcobalamin (E-GSCbl) containing fluorescent eosin-labeled glutathione (E-GSH) as the upper axial ligand to the cobalt. E-GSH fluorescence was strongly quenched in E-GSCbl. The E-GSH ligand of E-GSCbl was replaced specifically by cyanide, showing recovery of the E-GSH fluorescence. This profluorescent property of E-GSCbl enabled detection of cyanide in aqueous solutions, yielding a lower detection limit of 10 nM (0.26 μg L-1). Moreover E-GSH exhibited strong luminescence under UV-light that was quenched in E-GSCbl, and this allowed naked-eye detection of cyanide at concentrations as low as 100 nM. This study demonstrates that profluorescent E-GSCbl is a highly sensitive cyanide chemosensor that can detect nanomolar concentrations of cyanide.