Andreas Nilsson1, Aurelia Santoro2, Claudio Franceschi3, Fawzi Kadi4. 1. School of Health Sciences, Örebro University, Sweden. 2. Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy; Interdepartmental Center "L. Galvani" (CIG), University of Bologna, Bologna, Italy. 3. University of Bologna and Institute of Neurological Sciences, Bellaria Hospital, Bologna, Italy. 4. School of Health Sciences, Örebro University, Sweden. Electronic address: Fawzi.kadi@oru.se.
Abstract
BACKGROUND: N-linked enzymatic glycosylation modulates the function of proteins and contributes to development of age-related metabolic abnormalities. Whether physical activity (PA) is linked to a specific N-glycan profile and can offset detrimental links between N-glycans and metabolic risk profile has never been explored. The aim of the present study is to assess serum N-glycan profile in older women with different PA levels and metabolic risk status. MATERIALS AND METHODS: Components of the metabolic syndrome (MetS) and serum N-glycans analyzed using DSA-FACE technology were assessed in 109 older community-dwelling women (65-70 yrs). Ten peaks, each representing a unique N-glycan structure were detected. Moderate-to-vigorous PA (MVPA) was assessed objectively using accelerometry. All analyses were adjusted by covariates. RESULTS: Significantly elevated levels of NGA2FB (peak 2) and NA3F (peak 9) and lower level of the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3) were demonstrated in women with MetS compared to their healthier peers (p < 0.05). Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05). Interestingly, time spent in PA below the MVPA threshold was not linked to N-glycans. CONCLUSION: Novel links between PA behaviors and N-glycan profile are demonstrated in older adults, regardless of metabolic risk status. This proposed effect on N-glycans requires engagement in MVPA. This supports public health efforts to promote adherence to PA guidelines in older adults across different stages of disease prevention.
BACKGROUND: N-linked enzymatic glycosylation modulates the function of proteins and contributes to development of age-related metabolic abnormalities. Whether physical activity (PA) is linked to a specific N-glycan profile and can offset detrimental links between N-glycans and metabolic risk profile has never been explored. The aim of the present study is to assess serum N-glycan profile in older women with different PA levels and metabolic risk status. MATERIALS AND METHODS: Components of the metabolic syndrome (MetS) and serum N-glycans analyzed using DSA-FACE technology were assessed in 109 older community-dwelling women (65-70 yrs). Ten peaks, each representing a unique N-glycan structure were detected. Moderate-to-vigorous PA (MVPA) was assessed objectively using accelerometry. All analyses were adjusted by covariates. RESULTS: Significantly elevated levels of NGA2FB (peak 2) and NA3F (peak 9) and lower level of the α(1,6)-arm monogalactosylated (NG1(6)A2F) (peak 3) were demonstrated in women with MetS compared to their healthier peers (p < 0.05). Importantly, women adhering to the PA guideline of time in MVPA had a 10% and a 12% lower level of NA3 (peak 8) and NA4 (peak 10), respectively, compared to those less active even after adjustment by MetS and covariates (p < 0.05). Interestingly, time spent in PA below the MVPA threshold was not linked to N-glycans. CONCLUSION: Novel links between PA behaviors and N-glycan profile are demonstrated in older adults, regardless of metabolic risk status. This proposed effect on N-glycans requires engagement in MVPA. This supports public health efforts to promote adherence to PA guidelines in older adults across different stages of disease prevention.
Authors: Ana Cvetko; Massimo Mangino; Cristina Menni; Olga Gornik; Marko Tijardović; Domagoj Kifer; Mario Falchi; Toma Keser; Markus Perola; Tim D Spector; Gordan Lauc Journal: BMJ Open Diabetes Res Care Date: 2021-09