| Literature DB >> 3115829 |
A P Bradford1, A Aitken, F Beg, K G Cook, S J Yeaman.
Abstract
The 2-oxoglutarate dehydrogenase complex was succinylated using 2-oxo[5-14C]glutarate in the presence of N-ethylmaleimide to label the lipoic acid cofactor of the transuccinylase (E2) component. Following peptic digestion, 14C-lipoate-containing peptides were purified and subjected to automated Edman degradation and amino acid analysis. The amino acid sequence surrounding the lipoyllysine residue is reported.Entities:
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Year: 1987 PMID: 3115829 DOI: 10.1016/0014-5793(87)80221-1
Source DB: PubMed Journal: FEBS Lett ISSN: 0014-5793 Impact factor: 4.124