| Literature DB >> 3115775 |
R Rella1, C A Raia, M Pensa, F M Pisani, A Gambacorta, M De Rosa, M Rossi.
Abstract
An NAD+-dependent alcohol dehydrogenase (alcohol: NAD+ oxidoreductase, EC 1.1.1.1) was detected in cellular extracts of the extreme thermophilic archaebacterium Sulfolobus solfataricus. The enzyme was purified to homogeneity and shown to be a dimer with a native molecular mass of 71 kDa by sucrose gradient centrifugation and SDS electrophoresis. The enzyme has a broad substrate specificity that includes linear and branched primary alcohols, linear and cyclic secondary alcohols, linear and cyclic ketones and anisaldehyde. The enzyme has an extraordinary thermophilicity and a remarkable thermostability, and appears to have some properties and a structure different from those previously described for thermophilic alcohol dehydrogenases.Entities:
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Year: 1987 PMID： 3115775 DOI： 10.1111/j.1432-1033.1987.tb13361.x
Source DB: PubMed Journal: Eur J Biochem ISSN： 0014-2956