Yong Zhou1, Zhaohua Li2, Yinglu Ding1, Peng Zhang1, Jinqing Wang1. 1. Department of General Surgery, The Second Affiliated Hospital of Shandong University, Ji'nan City, Shandong Province, 250033, PR China. 2. Department of Medical Imaging, The Second Affiliated Hospital of Shandong University, Ji'nan City, Shandong Province, 250033, PR China. Electronic address: lqyex45@163.com.
Abstract
BACKGROUND: Increasing researches found crucial roles of microRNAs (miRNAs) in malignant tumor progression. In various cancers, miR-340 was low-expressed and served as an independent marker for predicting survival of patients with pancreatic cancer. However, the functions and molecular mechanisms of miRNA-340 in pancreatic cancer (PC) remain unclear. RESULTS: MiR-340 was downregulated in pancreatic cancer cell lines and tissues, and its high-expression suppressed pancreatic cancer cell growth. Then motor adaptor protein BICD2 was identified and proved as one target of miR-340, which was up-regulated in pancreatic cancer tissues and cell lines. However, knockdown of BICD2 significantly weakened cancer cell growth. In vivo experiments further confirmed that miR-340 restoration could reduce tumor size, Ki-67 positive ratio and BICD2 expression, indicating its anti-tumor effects. CONCLUSION: Altogether, these findings indicated that miR-340 exerted suppressor on cell growth by targeting BICD2 in pancreatic cancer and miR-340/BICD2 axis could be used as a candidate target to treat pancreatic cancer.
BACKGROUND: Increasing researches found crucial roles of microRNAs (miRNAs) in malignant tumor progression. In various cancers, miR-340 was low-expressed and served as an independent marker for predicting survival of patients with pancreatic cancer. However, the functions and molecular mechanisms of miRNA-340 in pancreatic cancer (PC) remain unclear. RESULTS:MiR-340 was downregulated in pancreatic cancer cell lines and tissues, and its high-expression suppressed pancreatic cancer cell growth. Then motor adaptor protein BICD2 was identified and proved as one target of miR-340, which was up-regulated in pancreatic cancer tissues and cell lines. However, knockdown of BICD2 significantly weakened cancer cell growth. In vivo experiments further confirmed that miR-340 restoration could reduce tumor size, Ki-67 positive ratio and BICD2 expression, indicating its anti-tumor effects. CONCLUSION: Altogether, these findings indicated that miR-340 exerted suppressor on cell growth by targeting BICD2 in pancreatic cancer and miR-340/BICD2 axis could be used as a candidate target to treat pancreatic cancer.