J Li1, X Chen1, X Lu1, C Zhang1, Q Shi1, L Feng1. 1. Jinan University, The First Affiliated Hospital, Department of Endocrinology, Guangzhou, China.
Abstract
CONTEXT: Peripheral nerve lesions are a major complication of diabetes mellitus, the main clinical manifestations of which are numbness and pain involving the limbs. OBJECTIVE: To determine the correlation between pregabalin treatment and diabetic peripheral neuropathic pain. DESIGN: An experimental animal study in BALB/c mice. SUBJECTS AND METHODS: Diabetes models are established by injecting streptozotocin (STZ) into the abdominal cavities of mice. The correlation between the treatment effect, time, and dosage of pregabalin was determined. The effect of a type 1 organic cation transporter (Octn1) in the absorption of pregabalin was evaluated. RESULTS: Pregabalin reduced tactile allodynia in diabetic mice. The best analgesic effect occurred when intestinal absorption was increased. Octn1 mediated pregabalin entry into intestinal epithelial cells, which influenced the absorption of pregabalin with a time-dependent fluctuation in the small intestine. Peripheral nerve damage caused by diabetes was dependent on time and dose of pregabalin, which was related to the regular expression of Octn1 in small intestinal epithelium. CONCLUSIONS: Peripheral nerve damage caused by diabetes was dependent on time and dosage of pregabalin, which was related to the regular expression of Octn1 in small intestinal epithelium.
CONTEXT: Peripheral nerve lesions are a major complication of diabetes mellitus, the main clinical manifestations of which are numbness and pain involving the limbs. OBJECTIVE: To determine the correlation between pregabalin treatment and diabetic peripheral neuropathic pain. DESIGN: An experimental animal study in BALB/c mice. SUBJECTS AND METHODS: Diabetes models are established by injecting streptozotocin (STZ) into the abdominal cavities of mice. The correlation between the treatment effect, time, and dosage of pregabalin was determined. The effect of a type 1 organic cation transporter (Octn1) in the absorption of pregabalin was evaluated. RESULTS: Pregabalin reduced tactile allodynia in diabetic mice. The best analgesic effect occurred when intestinal absorption was increased. Octn1 mediated pregabalin entry into intestinal epithelial cells, which influenced the absorption of pregabalin with a time-dependent fluctuation in the small intestine. Peripheral nerve damage caused by diabetes was dependent on time and dose of pregabalin, which was related to the regular expression of Octn1 in small intestinal epithelium. CONCLUSIONS: Peripheral nerve damage caused by diabetes was dependent on time and dosage of pregabalin, which was related to the regular expression of Octn1 in small intestinal epithelium.
Authors: K Bleasby; J C Castle; C J Roberts; C Cheng; W J Bailey; J F Sina; A V Kulkarni; M J Hafey; R Evers; J M Johnson; R G Ulrich; J G Slatter Journal: Xenobiotica Date: 2006 Oct-Nov Impact factor: 1.908
Authors: Klaus Fink; David J Dooley; Wolfgang P Meder; Nirmala Suman-Chauhan; Sandra Duffy; Hans Clusmann; Manfred Göthert Journal: Neuropharmacology Date: 2002-02 Impact factor: 5.250
Authors: Dirk Gründemann; Stephanie Harlfinger; Stefan Golz; Andreas Geerts; Andreas Lazar; Reinhard Berkels; Norma Jung; Andrea Rubbert; Edgar Schömig Journal: Proc Natl Acad Sci U S A Date: 2005-03-28 Impact factor: 11.205
Authors: Anne-Marie Lamhonwah; Jennifer Skaug; Stephen W Scherer; Ingrid Tein Journal: Biochem Biophys Res Commun Date: 2003-01-31 Impact factor: 3.575