Literature DB >> 31148045

Quantifying Plant Growth and Cell Proliferation with MorphoGraphX.

Soeren Strauss1, Aleksandra Sapala1,2, Daniel Kierzkowski1,3, Richard S Smith4.   

Abstract

Confocal microscopy is widely used to live-image plant tissue. Cell outlines can be visualized using fluorescent probes that mark the cell wall or plasma membrane, enabling the confocal microscope to be used as a 3D scanner with submicron precision. After imaging, the data needs to be analyzed by specialized software to quantify the features of interest, such as cell size and shape, growth rates and anisotropy, and gene expression. Here we present a protocol for the 3D image processing software MorphoGraphX ( www.MorphoGraphX.org ) using time-lapse images of an Arabidopsis thaliana sepal and the shoot apex of tomato.

Entities:  

Keywords:  3D visualization; Cell proliferation; Confocal microscopy; Growth analysis; Image analysis; Lineage tracking; Time lapse

Mesh：

Year:  2019        PMID： 31148045     DOI： 10.1007/978-1-4939-9469-4_18

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  Growth dynamics of the Arabidopsis fruit is mediated by cell expansion.

Authors:  Juan-José Ripoll; Mingyuan Zhu; Stephanie Brocke; Cindy T Hon; Martin F Yanofsky; Arezki Boudaoud; Adrienne H K Roeder
Journal:  Proc Natl Acad Sci U S A       Date:  2019-11-22       Impact factor: 11.205

Review 2.  Future Challenges in Plant Systems Biology.

Authors:  Mikaël Lucas
Journal:  Methods Mol Biol       Date:  2022

3.  Quantitative Live Confocal Imaging in AquilegiaFloral Meristems.

Authors:  Ya Min; Stephanie J Conway; Elena M Kramer
Journal:  Bio Protoc       Date:  2022-06-20

4.  Live-imaging provides an atlas of cellular growth dynamics in the stamen.

Authors:  Sylvia R Silveira; Constance Le Gloanec; Andrea Gómez-Felipe; Anne-Lise Routier-Kierzkowska; Daniel Kierzkowski
Journal:  Plant Physiol       Date:  2022-02-04       Impact factor: 8.005

5.  Using positional information to provide context for biological image analysis with MorphoGraphX 2.0.

Authors:  Sören Strauss; Adam Runions; Brendan Lane; Dennis Eschweiler; Namrata Bajpai; Nicola Trozzi; Anne-Lise Routier-Kierzkowska; Saiko Yoshida; Sylvia Rodrigues da Silveira; Athul Vijayan; Rachele Tofanelli; Mateusz Majda; Emillie Echevin; Constance Le Gloanec; Hana Bertrand-Rakusova; Milad Adibi; Kay Schneitz; George W Bassel; Daniel Kierzkowski; Johannes Stegmaier; Miltos Tsiantis; Richard S Smith
Journal:  Elife       Date:  2022-05-05       Impact factor: 8.713

6.  A digital 3D reference atlas reveals cellular growth patterns shaping the Arabidopsis ovule.

Authors:  Athul Vijayan; Rachele Tofanelli; Sören Strauss; Lorenzo Cerrone; Adrian Wolny; Joanna Strohmeier; Anna Kreshuk; Fred A Hamprecht; Richard S Smith; Kay Schneitz
Journal:  Elife       Date:  2021-01-06       Impact factor: 8.140

7.  Accurate and versatile 3D segmentation of plant tissues at cellular resolution.

Authors:  Adrian Wolny; Lorenzo Cerrone; Athul Vijayan; Rachele Tofanelli; Amaya Vilches Barro; Marion Louveaux; Christian Wenzl; Sören Strauss; David Wilson-Sánchez; Rena Lymbouridou; Susanne S Steigleder; Constantin Pape; Alberto Bailoni; Salva Duran-Nebreda; George W Bassel; Jan U Lohmann; Miltos Tsiantis; Fred A Hamprecht; Kay Schneitz; Alexis Maizel; Anna Kreshuk
Journal:  Elife       Date:  2020-07-29       Impact factor: 8.140

Review 8.  Imaging the living plant cell: From probes to quantification.

Authors:  Leia Colin; Raquel Martin-Arevalillo; Simone Bovio; Amélie Bauer; Teva Vernoux; Marie-Cecile Caillaud; Benoit Landrein; Yvon Jaillais
Journal:  Plant Cell       Date:  2022-01-20       Impact factor: 12.085

9.  Floral organ development goes live.

Authors:  Léa Rambaud-Lavigne; Angela Hay
Journal:  J Exp Bot       Date:  2020-05-09       Impact factor: 6.992
  9 in total

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