Literature DB >> 31147946

Monitoring MHC-II Endocytosis and Recycling Using Cell-Surface Protein Biotinylation-Based Assays.

Kyung-Jin Cho1, Paul A Roche2.   

Abstract

Most, if not all, plasma membrane proteins continuously undergo endocytosis and many rapidly recycle from endosomes back to the cell surface to maintain "stable" surface expression. We now describe a biochemical assay that is suited to follow the internalization and recycling kinetics of plasma membrane proteins. This assay involves biotinylation of plasma membrane proteins using sulfo-NHS-SS-biotin, a water-soluble, NHS-ester biotinylation reagent that contains a cleavable disulfide bond that allows for reversible labeling of proteins. Biotinylation is rapid and stable, and does not transfer from cell to cell, and the small size of the biotin probe does not affect cell function.

Entities:  

Keywords:  Biotinylation; Endocytosis; Glutathione; Immunoblot; MHC class II; Recycling

Mesh：

Substances：

Year:  2019        PMID： 31147946      PMCID： PMC8259318          DOI： 10.1007/978-1-4939-9450-2_19

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  Ubiquitination regulates MHC class II-peptide complex retention and degradation in dendritic cells.

Authors:  Even Walseng; Kazuyuki Furuta; Berta Bosch; Karis A Weih; Yohei Matsuki; Oddmund Bakke; Satoshi Ishido; Paul A Roche
Journal:  Proc Natl Acad Sci U S A       Date:  2010-11-08       Impact factor: 11.205

Review 2.  The ins and outs of MHC class II-mediated antigen processing and presentation.

Authors:  Paul A Roche; Kazuyuki Furuta
Journal:  Nat Rev Immunol       Date:  2015-02-27       Impact factor: 53.106

3.  Ubiquitination by March-I prevents MHC class II recycling and promotes MHC class II turnover in antigen-presenting cells.

Authors:  Kyung-Jin Cho; Even Walseng; Satoshi Ishido; Paul A Roche
Journal:  Proc Natl Acad Sci U S A       Date:  2015-08-03       Impact factor: 11.205

4.  MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation.

Authors:  Aude De Gassart; Voahirana Camosseto; Jacques Thibodeau; Maurizio Ceppi; Nadia Catalan; Philippe Pierre; Evelina Gatti
Journal:  Proc Natl Acad Sci U S A       Date:  2008-02-27       Impact factor: 11.205

Review 5.  Regulation of MHC Class II-Peptide Complex Expression by Ubiquitination.

Authors:  Kyung-Jin Cho; Paul A Roche
Journal:  Front Immunol       Date:  2013-11-13       Impact factor: 7.561
  5 in total
  1 in total

1.  Factor H Is Bound by Outer Membrane-Displayed Carbohydrate Metabolism Enzymes of Extraintestinal Pathogenic Escherichia coli and Contributes to Opsonophagocytosis Resistance in Bacteria.

Authors:  Yu Sun; Bin Xu; Xiangkai Zhuge; Fang Tang; Xuhang Wang; Qianwen Gong; Rui Chen; Feng Xue; Jianjun Dai
Journal:  Front Cell Infect Microbiol       Date:  2021-01-25       Impact factor: 5.293
  1 in total

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