| Literature DB >> 31147937 |
Annika Nelde1, Daniel J Kowalewski1, Stefan Stevanović2.
Abstract
The large-scale and in-depth identification of MHC class I- and II-presented peptides is indispensable for gaining insight into the fundamental rules of immune recognition as well as for developing innovative immunotherapeutic approaches against cancer and other diseases. In this chapter we briefly review the existing strategies for the isolation of MHC-restricted peptides and provide a detailed protocol for the immunoaffinity purification of MHC class I- and II-presented peptides from primary tissues or cells.Entities:
Keywords: Antigen presentation; Human leukocyte antigen (HLA); Immunoaffinity purification; Immunopeptidomics; Ligandome analysis; Major histocompatibility complex (MHC); Mass spectrometry
Year: 2019 PMID： 31147937 DOI： 10.1007/978-1-4939-9450-2_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745