Huiyi Song1, Jianbin Zhang2, Jialin Qu1, Jianjun Liu1, Peiyuan Yin1, Guixin Zhang3,4, Dong Shang5,6. 1. Clinical Laboratory of Integrative Medicine, First Hospital Affiliated to Dalian Medical University, 222 Zhongshan Road, Dalian, 116023, Liaoning, People's Republic of China. 2. College of Pharmacy, Dalian Medical University, Dalian, Liaoning, People's Republic of China. 3. Clinical Laboratory of Integrative Medicine, First Hospital Affiliated to Dalian Medical University, 222 Zhongshan Road, Dalian, 116023, Liaoning, People's Republic of China. zgx0109@126.com. 4. The Third Department of General Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, People's Republic of China. zgx0109@126.com. 5. Clinical Laboratory of Integrative Medicine, First Hospital Affiliated to Dalian Medical University, 222 Zhongshan Road, Dalian, 116023, Liaoning, People's Republic of China. shangdong@dmu.edu.cn. 6. The Third Department of General Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, People's Republic of China. shangdong@dmu.edu.cn.
Abstract
OBJECTIVES: Microbial biofilms have become one of the most significant causes of nosocomial infections. The aim of this study was to examine the potential quorum sensing inhibitor activities of Lactobacillus rhamnosus GG microcapsules. RESULTS: Lactobacillus rhamnosus GG microcapsules effectively inhibited initial biofilm formation at a concentration of 2.5 × 108 CFU/mL. Furthermore, the inhibition rate was increased to 79% in the Lactobacillus rhamnosus GG microcapsules group, resulting in a reduction in the biofilm maturation stage. In addition, real-time PCR analysis revealed that the LGG microcapsules can act as effective inhibitors of transcriptional activators of the quorum sensing circuit in E.coli, luxS, lsrK, and lsrR. CONCLUSIONS: Lactobacillus rhamnosus GG microcapsules can effectively inhibit biofilm formation and disturb mature biofilms.
OBJECTIVES: Microbial biofilms have become one of the most significant causes of nosocomial infections. The aim of this study was to examine the potential quorum sensing inhibitor activities of Lactobacillus rhamnosus GG microcapsules. RESULTS:Lactobacillus rhamnosus GG microcapsules effectively inhibited initial biofilm formation at a concentration of 2.5 × 108 CFU/mL. Furthermore, the inhibition rate was increased to 79% in the Lactobacillus rhamnosus GG microcapsules group, resulting in a reduction in the biofilm maturation stage. In addition, real-time PCR analysis revealed that the LGG microcapsules can act as effective inhibitors of transcriptional activators of the quorum sensing circuit in E.coli, luxS, lsrK, and lsrR. CONCLUSIONS:Lactobacillus rhamnosus GG microcapsules can effectively inhibit biofilm formation and disturb mature biofilms.
Entities:
Keywords:
Biofilm inhibition; Coculture model; Probiotic microcapsules; Quorum sensing
Authors: Agustina Rodríguez-Sorrento; Lorena Castillejos; Paola López-Colom; Gloria Cifuentes-Orjuela; María Rodríguez-Palmero; José Antonio Moreno-Muñoz; Diana Luise; Paolo Trevisi; Susana María Martín-Orúe Journal: Front Microbiol Date: 2021-04-16 Impact factor: 5.640