| Literature DB >> 31146867 |
Anjaly N Vijayan1, Zhiming Liu2, Haohan Zhao1, Peng Zhang3.
Abstract
A highly specific and sensitive isothermal method for mercury detection using DNA-conjugated upconversion nanoparticles is reported. A single-stranded DNA containing thymine bases, used as the Hg2+-capturing element through the formation of thymine-Hg2+-thymine complex, is covalently attached to the NaYF4: Yb3+, Tm3+ nanoparticles. Luminescence resonance energy transfer takes place between the NaYF4: Yb3+, Tm3+ nanoparticles as donor and DNA-intercalating SYBR Green I as the acceptor upon excitation of 980 nm. The sensitivity and selectivity toward Hg2+ are enhanced using the nicking enzyme, Nt. Alwl, which leads to signal amplification. By monitoring the ratio of acceptor emission to a reference peak, the presence of Hg2+ ions are quantitatively determined with a lower detection limit of 0.14 nM, which is much lower than the US Environmental Protection Agency (EPA) limit of Hg2+ in drinking water.Entities:
Keywords: Luminescence resonance energy transfer (LRET); Mercuric ion; Nicking enzyme; Signal amplification; Upconversion nanoparticles (UCNPs)
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Year: 2019 PMID： 31146867 PMCID： PMC6546430 DOI： 10.1016/j.aca.2019.05.001
Source DB: PubMed Journal: Anal Chim Acta ISSN： 0003-2670 Impact factor: 6.558