Infectivity of cloned begomoviral DNAs: an appraisal.

Infectivity of cloned begomoviral DNAs is an important criterion to establish the etiology of the disease it causes, to study viral gene functions and host-virus interactions. Three main methods have been employed to study infectivity; mechanical inoculation with cloned viral DNA using abrasives, Agrobacterium-mediated inoculation (agroinoculation) of cloned viral DNA and bombardment using microprojectiles coated with cloned viral DNA (biolistics). Each method has its own advantages and disadvantages and the adoption of one over the other for demonstrating infectivity depends on various factors. This review compares the various features associated with the above three methods.