Guanine nucleotide regulatory proteins as transducers of receptor-stimulated neutrophil activation.

There exists a family of homologous GTP-binding regulatory proteins (the G proteins) which couple receptors to cellular effector systems. Several lines of evidence indicate a major role for a G protein in mediating neutrophil activation by a variety of chemotactic factors. We here describe studies on guanine nucleotide regulation of formyl peptide receptor affinity in a permeabilized neutrophil preparation. The characterization of neutrophil G protein with anti-G protein antibodies is also discussed.