Ana C Anauate1, Mariana F Leal1, Fernanda Wisnieski1,2, Leonardo C Santos1, Carolina O Gigek1,3, Elizabeth S Chen1, Danielle Q Calcagno4, Paulo P Assumpção4, Sâmia Demachki4, Carlos H Arasaki3, Ricardo Artigiani5, Lucas T Rasmussen6, Spencer M Payão6, Rommel R Burbano4,7,8, Marília A C Smith1. 1. Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, São Paulo, 04023-900, Brazil. 2. Disciplina de Gastroenterologia, Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, 04040-002, Brazil. 3. Disciplina de Gastroenterologia Cirúrgica, Departamento de Cirurgia, Universidade Federal de São Paulo, São Paulo, 04024-002, Brazil. 4. Núcleo de Pesquisas em Oncologia, Hospital Universitário João de Barros Barreto, Universidade Federal do Pará, Belém, 66073-000, Brazil. 5. Departamento de Patologia, Universidade Federal de São Paulo, São Paulo, 04023-062, Brazil. 6. Departamento de Genética e Biologia Molecular, Faculdade de Medicina de Marília, Marília, 17519-050, Brazil. 7. Laboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, 66075-110, Brazil. 8. Laboratório de Biologia Molecular, Hospital Ophir Loyola, Belém, 66063-240, Brazil.
Abstract
Aim: To analyze gene expression and copy number of five miRNAs (miR-1204, miR-1205, miR-1206, miR-1207 and miR-1208) localized in this chromosome region in gastric cancer (GC). Materials & methods: 65 paired neoplastic and non-neoplastic specimens collected from GC patients and 20 non-neoplastic gastric tissues from cancer-free individuals were included in this study. The expression levels of the five miRNAs were accessed by real time qPCR and were correlated. Results: MiR-1207-3p, miR-1205, miR-1207-5p and miR-1208 were upregulated in approximately 50% of GC tumors in relation to those of adjacent non-neoplastic tissues. MiR-1205 expression was associated with gain of gene copies and was upregulated in adjacent non-neoplastic samples relative to external controls. Conclusion: The coexpression of the 8q24 miRNAs indicated the role of miR-1205 in the initiation of gastric cancer development.
Aim: To analyze gene expression and copy number of five miRNAs (miR-1204, miR-1205, miR-1206, miR-1207 and miR-1208) localized in this chromosome region in gastric cancer (GC). Materials & methods: 65 paired neoplastic and non-neoplastic specimens collected from GC patients and 20 non-neoplastic gastric tissues from cancer-free individuals were included in this study. The expression levels of the five miRNAs were accessed by real time qPCR and were correlated. Results:MiR-1207-3p, miR-1205, miR-1207-5p and miR-1208 were upregulated in approximately 50% of GC tumors in relation to those of adjacent non-neoplastic tissues. MiR-1205 expression was associated with gain of gene copies and was upregulated in adjacent non-neoplastic samples relative to external controls. Conclusion: The coexpression of the 8q24 miRNAs indicated the role of miR-1205 in the initiation of gastric cancer development.