Guilherme Ferreira da Silva1, Francine Cesário2, Alan Matheus Ramos Garcia3, Paulo Henrique Weckwerth3, Marco Antônio Húngaro Duarte2, Rodrigo Cardoso de Oliveira4, Rodrigo Ricci Vivan2. 1. Pro-Rectory of Research and Post-Graduation, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, SP, Brazil. gferreiras@hotmail.com. 2. Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil. 3. Pro-Rectory of Research and Post-Graduation, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, SP, Brazil. 4. Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
Abstract
OBJECTIVES: Evaluate the cytotoxicity and biocompatibility of calcium hydroxide paste [Ca (OH)2] associated with 5% diclofenac sodium, ibuprofen, or amoxicillin. MATERIALS AND METHODS: Pre-osteoblast-like cells were cultivated and the MTT test was used to determine the cytotoxicity of the paste extracts after time intervals of 24, 48, 72 h, and 7 days. Tubes containing Ca (OH)2 pastes associated with the drugs and empty tubes were implanted in subcutaneous tissue of 30 rats. After 7 and 30 days, the specimens were removed and submitted to histological analysis. The data obtained were submitted to Kruskal-Wallis and Dunn tests (p ≤ 0.05). RESULTS: All the Ca (OH)2 pastes promoted cell viability after all periods. At 7 days, there was greater inflammatory tissue reaction adjacent to the implants. At 30 days, there was a significant reduction in the number of inflammatory cells, and increase in fibroblasts in all groups. In this period, a lower number of inflammatory cells and a higher number of fibroblasts were observed in the capsules adjacent to the association with diclofenac, when compared with the other mixtures (p ≤ 0.05); the capsule thickness was greater at 7 days than at 30 days. CONCLUSIONS: The Ca (OH)2 pastes associated with the drugs were not cytotoxic and presented biocompatibility after implantation in rat subcutaneous tissues. CLINICAL RELEVANCE: Ca (OH)2 pastes with anti-inflammatory or antibiotic may be clinical alternatives as intracanal medication to reduce resistant microorganisms in root canal system.
OBJECTIVES: Evaluate the cytotoxicity and biocompatibility of calcium hydroxide paste [Ca (OH)2] associated with 5% diclofenac sodium, ibuprofen, or amoxicillin. MATERIALS AND METHODS: Pre-osteoblast-like cells were cultivated and the MTT test was used to determine the cytotoxicity of the paste extracts after time intervals of 24, 48, 72 h, and 7 days. Tubes containing Ca (OH)2 pastes associated with the drugs and empty tubes were implanted in subcutaneous tissue of 30 rats. After 7 and 30 days, the specimens were removed and submitted to histological analysis. The data obtained were submitted to Kruskal-Wallis and Dunn tests (p ≤ 0.05). RESULTS: All the Ca (OH)2 pastes promoted cell viability after all periods. At 7 days, there was greater inflammatory tissue reaction adjacent to the implants. At 30 days, there was a significant reduction in the number of inflammatory cells, and increase in fibroblasts in all groups. In this period, a lower number of inflammatory cells and a higher number of fibroblasts were observed in the capsules adjacent to the association with diclofenac, when compared with the other mixtures (p ≤ 0.05); the capsule thickness was greater at 7 days than at 30 days. CONCLUSIONS: The Ca (OH)2 pastes associated with the drugs were not cytotoxic and presented biocompatibility after implantation in rat subcutaneous tissues. CLINICAL RELEVANCE: Ca (OH)2 pastes with anti-inflammatory or antibiotic may be clinical alternatives as intracanal medication to reduce resistant microorganisms in root canal system.
Authors: P Montero-Miralles; J Martín-González; O Alonso-Ezpeleta; M C Jiménez-Sánchez; E Velasco-Ortega; J J Segura-Egea Journal: Int Endod J Date: 2018-03-13 Impact factor: 5.264
Authors: Marta E Valverde; Pilar Baca; Laura Ceballos; M Victoria Fuentes; Matilde Ruiz-Linares; Carmen María Ferrer-Luque Journal: Dent Mater J Date: 2017-02-22 Impact factor: 2.102
Authors: Flávia A Oliveira; Adriana A Matos; Mariana R Santesso; Cintia K Tokuhara; Aline L Leite; Vanderley S Bagnato; Maria A A M Machado; Camila Peres-Buzalaf; Rodrigo C Oliveira Journal: J Photochem Photobiol B Date: 2016-08-06 Impact factor: 6.252