| Literature DB >> 31138766 |
Weiliang Fan1,2, Jia Guo1,2, Beichen Gao1,2, Wenbin Zhang1,2, Liucong Ling1,2, Tao Xu1,2, Chenjie Pan1,2, Lin Li1,2, She Chen1,2, Hua Wang3,4, Jing Zhang3,5, Xiaodong Wang6,2.
Abstract
Necroptosis is a form of regulated necrosis that is implicated in various human diseases including Alzheimer's disease. Necroptosis requires the translocation of the pseudokinase MLKL from the cytosol to the plasma membrane after its phosphorylation by the kinase RIPK3. Using protein cross-linking followed by affinity purification, we detected the lipid raft-associated proteins flotillin-1 and flotillin-2 and the ESCRT-associated proteins ALIX and syntenin-1 in membrane-localized MLKL immunoprecipitates. Phosphorylated MLKL was removed from membranes through either flotillin-mediated endocytosis followed by lysosomal degradation or ALIX-syntenin-1-mediated exocytosis. Thus, cells undergoing necroptosis need to overcome these independent suppressive mechanisms before plasma membrane disruption can occur.Entities:
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Year: 2019 PMID： 31138766 DOI： 10.1126/scisignal.aaw3423
Source DB: PubMed Journal: Sci Signal ISSN： 1945-0877 Impact factor: 8.192