Jun Zheng1,2, Fazhan Wang1,3, Yongyong Yang4, Jiang Xu1,2, Jinhua Yang5, Keying Wang1,2, Yuhao Liu1, Gang Du5,6, Yan Zeng1,3. 1. Key Laboratory of Xinjiang Endemic and Ethnic Disease, School of Medicine, Shihezi University, Shihezi, China. 2. Department of Stomatology, The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China. 3. Department of Biochemistry, School of Medicine, Shihezi University, Shihezi, China. 4. Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital & Institute, Beijing, China. 5. Department of Bioinformatics, Guangzhou GenCoding Lab, Guangzhou, China. 6. Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Abstract
BACKGROUND: This study aims to explore the associations between N-α-acetyltransferase 10 protein (Naa10p) and p53-induced protein with a RING-H2 domain (Pirh2) expression and clinicopathological characteristics in oral squamous cell carcinoma (OSCC). METHODS: Immunohistochemistry was performed to detect Naa10p and Pirh2 levels containing 118 OSCC specimens, and additional analyses were used to determine correlations between Naa10p and Pirh2 expressions, generate survival curves, and perform univariate and multivariate statistical analyses. Further, quantitative real-time PCR (qRT-PCR) and western blot were employed to examine Naa10p and Pirh2 expression level in OSCC patients' samples. We further validated the result using RNAseq data from The Cancer Genome Atlas (TCGA) and mRNA array data from GSE31056 and GSE30784. RESULTS: Naa10p and Pirh2 are overexpression, and the protein level of Naa10p was negatively correlated with that of Pirh2 in OSCC tissues. Multivariate Cox proportional hazard regression analysis showed that positive Naa10p expression and negative Pirh2 expression were both independent good prognostic factors for OSCC patients. Furthermore, the Naa10p-positive/Pirh2-negative group has the best prognosis among all OSCC patients. Results from qRT-PCR showed the higher expression level of Naa10 and lower expression level of Pirh2 in tumor tissues than adjacent normal tissues. TCGA database and data from GSE31056 and GSE30784 showed the similar result. The correlation analysis showed that the mRNA level of Naa10 was negatively correlated that of Pirh2. CONCLUSION: The expression of Naa10p is negatively correlated with that of Pirh2, and positive Naa10p and negative Pirh2 might be independent biomarkers for better OSCC prognoses.
BACKGROUND: This study aims to explore the associations between N-α-acetyltransferase 10 protein (Naa10p) and p53-induced protein with a RING-H2 domain (Pirh2) expression and clinicopathological characteristics in oral squamous cell carcinoma (OSCC). METHODS: Immunohistochemistry was performed to detect Naa10p and Pirh2 levels containing 118 OSCC specimens, and additional analyses were used to determine correlations between Naa10p and Pirh2 expressions, generate survival curves, and perform univariate and multivariate statistical analyses. Further, quantitative real-time PCR (qRT-PCR) and western blot were employed to examine Naa10p and Pirh2 expression level in OSCC patients' samples. We further validated the result using RNAseq data from The Cancer Genome Atlas (TCGA) and mRNA array data from GSE31056 and GSE30784. RESULTS:Naa10p and Pirh2 are overexpression, and the protein level of Naa10p was negatively correlated with that of Pirh2 in OSCC tissues. Multivariate Cox proportional hazard regression analysis showed that positive Naa10p expression and negative Pirh2 expression were both independent good prognostic factors for OSCC patients. Furthermore, the Naa10p-positive/Pirh2-negative group has the best prognosis among all OSCC patients. Results from qRT-PCR showed the higher expression level of Naa10 and lower expression level of Pirh2 in tumor tissues than adjacent normal tissues. TCGA database and data from GSE31056 and GSE30784 showed the similar result. The correlation analysis showed that the mRNA level of Naa10 was negatively correlated that of Pirh2. CONCLUSION: The expression of Naa10p is negatively correlated with that of Pirh2, and positive Naa10p and negative Pirh2 might be independent biomarkers for better OSCC prognoses.
Keywords:
N-α-acetyltransferase 10 protein; The Cancer Genome Atlas; oral squamous cell carcinoma; p53-induced protein with a RING-H2 domain; prognosis