Anna-Katariina Aatsinki1, Leo Lahti2, Henna-Maria Uusitupa3, Eveliina Munukka4, Anniina Keskitalo5, Saara Nolvi6, Siobhain O'Mahony7, Sami Pietilä8, Laura L Elo9, Erkki Eerola10, Hasse Karlsson11, Linnea Karlsson12. 1. The FinnBrain Birth Cohort Study, Turku Brain and Mind Center, Department of Clinical, Medicine, University of Turku, Lemminkäisenkatu 3 a, Teutori Building, 20014 Turun yliopisto, Finland. Electronic address: ankaaa@utu.fi. 2. Department of Mathematics and Statistics, University of Turku, Quantum, Vesilinnantie 5, 20014 Turku, Finland. Electronic address: leo.lahti@utu.fi. 3. The FinnBrain Birth Cohort Study, Turku Brain and Mind Center, Department of Clinical, Medicine, University of Turku, Lemminkäisenkatu 3 a, Teutori Building, 20014 Turun yliopisto, Finland. Electronic address: hemauu@utu.fi. 4. Faculty of Medicine, University of Turku, Medisiina D, 7(th) floor, Kiinanmyllynkatu 10, 20520 Turku, Finland; Department of Clinical Microbiology and Immunology, Turku University Hospital and University of Turku, Medisiina D, 7(th) Floor, Kiinanmyllynkatu 10, 20520 Turku, Finland. Electronic address: laevmu@utu.fi. 5. Department of Clinical Microbiology and Immunology, Turku University Hospital and University of Turku, Medisiina D, 7(th) Floor, Kiinanmyllynkatu 10, 20520 Turku, Finland; Institute of Biomedicine, University of Turku, Medisiina D, 7(th) Floor, Kiinanmyllynkatu 10, 20520 Turku, Finland. Electronic address: anniina.keskitalo@utu.fi. 6. The FinnBrain Birth Cohort Study, Turku Brain and Mind Center, Department of Clinical, Medicine, University of Turku, Lemminkäisenkatu 3 a, Teutori Building, 20014 Turun yliopisto, Finland. Electronic address: saara.nolvi@utu.fi. 7. Department of Anatomy and Neuroscience, and APC Microbiome Ireland, Biosciences Building, University College Cork, Ireland. Electronic address: SOMahony@ucc.ie. 8. Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Tykistökatu 6, 20520 Turku, Finland. Electronic address: sami.pietila@utu.fi. 9. Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Tykistökatu 6, 20520 Turku, Finland. Electronic address: laura.elo@utu.fi. 10. Institute of Biomedicine, University of Turku, Medisiina D, 7(th) Floor, Kiinanmyllynkatu 10, 20520 Turku, Finland. Electronic address: eerola@utu.fi. 11. The FinnBrain Birth Cohort Study, Turku Brain and Mind Center, Department of Clinical, Medicine, University of Turku, Lemminkäisenkatu 3 a, Teutori Building, 20014 Turun yliopisto, Finland; Department of Psychiatry, Turku University Hospital and University of Turku, Kiinamyllynkatu 4-8, 20520 Turku, Finland. Electronic address: hasse.karlsson@utu.fi. 12. The FinnBrain Birth Cohort Study, Turku Brain and Mind Center, Department of Clinical, Medicine, University of Turku, Lemminkäisenkatu 3 a, Teutori Building, 20014 Turun yliopisto, Finland; Department of Child Psychiatry, Turku University Hospital and University of Turku, Kiinamyllynkatu 4-8, 20520 Turku, Finland. Electronic address: linnea.karlsson@utu.fi.
Abstract
BACKGROUND: One of the key behavioral phenotypes in infancy are different temperament traits, and certain early life temperament traits have been shown to precede later mental health problems. Differences in the gut microbiota composition (GMC) have been suggested to link with neurodevelopment. For example, toddler temperament traits have been found to associate with differences in GMC; however, studies in infants are lacking although infancy is a rapid period of neurodevelopment as well as GM development. Thus, we aimed to investigate association between infant GMC and temperament. METHODS: The study population (n = 301, 53% boys) was drawn from the FinnBrain Birth Cohort Study. Stool samples were collected from the 2.5-month-old infants and sequenced with 16S Illumina MiSeq platform. GMC taxonomic composition (at Genus and OTU level), observed sample clusters, diversity and richness were investigated in relation to the maternal reports of Infant Behavior Questionnaire -Revised (IBQ-R) at the age of 6 months. RESULTS: Three sample clusters (Bifidobacterium/Enterobacteriaceae, Bacteroides, V. Dispar) based on GMC were identified, of which Bifidobacterium/Enterobacteriaceae-cluster presented with higher scores on the IBQ-R main dimension regulation and its subscale duration of orienting compared to Bacteroides-cluster. The clusters associated with temperament in a sex-dependent manner. The IBQ-R main dimension surgency (positive emotionality) was associated positively both with genus Bifidobacterium and Streptococcus. Alpha diversity had a negative association with negative emotionality and fear reactivity. CONCLUSION: This is the first study demonstrating associations, but not causal connections, between GMC and temperament in young infants in a prospective design.
BACKGROUND: One of the key behavioral phenotypes in infancy are different temperament traits, and certain early life temperament traits have been shown to precede later mental health problems. Differences in the gut microbiota composition (GMC) have been suggested to link with neurodevelopment. For example, toddler temperament traits have been found to associate with differences in GMC; however, studies in infants are lacking although infancy is a rapid period of neurodevelopment as well as GM development. Thus, we aimed to investigate association between infant GMC and temperament. METHODS: The study population (n = 301, 53% boys) was drawn from the FinnBrain Birth Cohort Study. Stool samples were collected from the 2.5-month-old infants and sequenced with 16S Illumina MiSeq platform. GMC taxonomic composition (at Genus and OTU level), observed sample clusters, diversity and richness were investigated in relation to the maternal reports of Infant Behavior Questionnaire -Revised (IBQ-R) at the age of 6 months. RESULTS: Three sample clusters (Bifidobacterium/Enterobacteriaceae, Bacteroides, V. Dispar) based on GMC were identified, of which Bifidobacterium/Enterobacteriaceae-cluster presented with higher scores on the IBQ-R main dimension regulation and its subscale duration of orienting compared to Bacteroides-cluster. The clusters associated with temperament in a sex-dependent manner. The IBQ-R main dimension surgency (positive emotionality) was associated positively both with genus Bifidobacterium and Streptococcus. Alpha diversity had a negative association with negative emotionality and fear reactivity. CONCLUSION: This is the first study demonstrating associations, but not causal connections, between GMC and temperament in young infants in a prospective design.
Authors: Molly Fox; S Melanie Lee; Kyle S Wiley; Venu Lagishetty; Curt A Sandman; Jonathan P Jacobs; Laura M Glynn Journal: Dev Psychopathol Date: 2021-06-10
Authors: Q Leyrolle; F Decoeur; G Briere; C Amadieu; A R A A Quadros; I Voytyuk; C Lacabanne; A Benmamar-Badel; J Bourel; A Aubert; A Sere; F Chain; L Schwendimann; B Matrot; T Bourgeois; S Grégoire; J G Leblanc; A De Moreno De Leblanc; P Langella; G R Fernandes; L Bretillon; C Joffre; R Uricaru; P Thebault; P Gressens; J M Chatel; S Layé; A Nadjar Journal: Neuropsychopharmacology Date: 2020-08-11 Impact factor: 7.853
Authors: Amy Loughman; Anne-Louise Ponsonby; Martin O'Hely; Christos Symeonides; Fiona Collier; Mimi L K Tang; John Carlin; Sarath Ranganathan; Katrina Allen; Angela Pezic; Richard Saffery; Felice Jacka; Leonard C Harrison; Peter D Sly; Peter Vuillermin Journal: EBioMedicine Date: 2020-02-12 Impact factor: 8.143