Yuan Gao1, Jianping Chen. 1. Department of Digestive Diseases, The First People's Hospital of Changzhou, the Third Affiliated Hospital of Soochow University, No. 185 Juqian Rd, Changzhou, 213003, Jiangsu, China.
Abstract
PURPOSE: This study aimed to investigate the regulatory effect of long non-coding RNA (lncRNA) NBAT-1 (neuroblastoma associated transcript 1) on the development and prognosis of gastric cancer (GC), and its underlying mechanism. METHODS: NBAT-1 expression in GC tissues, paracancerous tissues and normal gastric tissues was detected by quantitative real-time polymerase chain reaction (qRT-PCR). We also detected NBAT-1 expression in GC cell lines. Correlation between NBAT-1 expression and TNM stage was analyzed. Overexpression plasmid of NBAT-1 was transfected into SGC-7901 and MGC-823 cells, followed by detection of proliferation and cell cycle by CCK-8 (cell counting kit-8) assay and flow cytometry. NBAT-1 localization in GC cells was accessed by chromatin fractionation. Finally, PTEN expression in GC cells overexpressing NBAT-1 was determined by Western blot analysis. RESULTS: NBAT-1 and PTEN were lowly expressed in GC tissues compared with paracancerous tissues and normal gastric tissues. GC patients with stage Ⅲ-Ⅳ presented lower expression of NBAT-1 than those with stage Ⅰ-Ⅱ. Besides, lower expression of NBAT-1 was found in GC patients with N2-N3 compared with those with N0-N1. NBAT-1 expression was not correlated with TNM stage and distant metastasis in GC patients. Upregulation of NBAT-1 in GC cells inhibited proliferation and arrested cell cycle. Chromatin fractionation results indicated that NBAT-1 was mainly localized in the cytoplasm of SGC-7901 and MGC-823 cells. NBAT-1 overexpression remarkably upregulated PTEN expression in GC cells. CONCLUSIONS: Low expression of NBAT-1 promotes GC development by downregulating PTEN expression.
PURPOSE: This study aimed to investigate the regulatory effect of long non-coding RNA (lncRNA) NBAT-1 (neuroblastoma associated transcript 1) on the development and prognosis of gastric cancer (GC), and its underlying mechanism. METHODS:NBAT-1 expression in GC tissues, paracancerous tissues and normal gastric tissues was detected by quantitative real-time polymerase chain reaction (qRT-PCR). We also detected NBAT-1 expression in GC cell lines. Correlation between NBAT-1 expression and TNM stage was analyzed. Overexpression plasmid of NBAT-1 was transfected into SGC-7901 and MGC-823 cells, followed by detection of proliferation and cell cycle by CCK-8 (cell counting kit-8) assay and flow cytometry. NBAT-1 localization in GC cells was accessed by chromatin fractionation. Finally, PTEN expression in GC cells overexpressing NBAT-1 was determined by Western blot analysis. RESULTS:NBAT-1 and PTEN were lowly expressed in GC tissues compared with paracancerous tissues and normal gastric tissues. GC patients with stage Ⅲ-Ⅳ presented lower expression of NBAT-1 than those with stage Ⅰ-Ⅱ. Besides, lower expression of NBAT-1 was found in GC patients with N2-N3 compared with those with N0-N1. NBAT-1 expression was not correlated with TNM stage and distant metastasis in GC patients. Upregulation of NBAT-1 in GC cells inhibited proliferation and arrested cell cycle. Chromatin fractionation results indicated that NBAT-1 was mainly localized in the cytoplasm of SGC-7901 and MGC-823 cells. NBAT-1 overexpression remarkably upregulated PTEN expression in GC cells. CONCLUSIONS: Low expression of NBAT-1 promotes GC development by downregulating PTEN expression.