Seyedeh-Sara Hashemi1, Ali Akbar Mohammadi1,2, Hamed Kabiri2, Mohammad Reza Hashempoor1, Mahdokht Mahmoodi1, Masood Amini3, Davood Mehrabani1,4. 1. Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 2. Division of Plastic and Reconstructive Surgery, Department of Surgery, Shiraz University of Medical Sciences, Shiraz, Iran. 3. Minimal Invasive Laparascopy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 4. Rohan Gene Cell Tech Center, Shiraz, Iran.
Abstract
BACKGROUND: Chronic wound or nonhealing ulcer is essentially a wound that does not progress normally through the wound healing process. This study assessed the healing effect of umbilical cord Wharton's jelly stem cells seeded on biological scaffold in chronic skin ulcers. MATERIALS AND METHODS: In a randomized clinical trial, five patients between 30 and 60 years with chronic diabetic wounds were enrolled. To cover the wounds, acellular amniotic membrane seeded with Wharton's jelly mesenchymal stem cells (WJSCs) was used for 9 days, every 3 days with a follow-up of 1 month. The percentage and time of wound healing and the size of wound were recorded for each patient. RESULTS: In treated patients, the wound healing time and wound size significantly decreased, and after 6 and 9 days, the wound size significantly declined (P < 0.002). CONCLUSION: As WJSCs seeded on amniotic membrane could significantly accelerate the healing effect in chronic diabetic wounds, they can be an alternative source in tissue engineering and repair of chronic ulcers.
RCT Entities:
BACKGROUND: Chronic wound or nonhealing ulcer is essentially a wound that does not progress normally through the wound healing process. This study assessed the healing effect of umbilical cord Wharton's jelly stem cells seeded on biological scaffold in chronic skin ulcers. MATERIALS AND METHODS: In a randomized clinical trial, five patients between 30 and 60 years with chronic diabetic wounds were enrolled. To cover the wounds, acellular amniotic membrane seeded with Wharton's jelly mesenchymal stem cells (WJSCs) was used for 9 days, every 3 days with a follow-up of 1 month. The percentage and time of wound healing and the size of wound were recorded for each patient. RESULTS: In treated patients, the wound healing time and wound size significantly decreased, and after 6 and 9 days, the wound size significantly declined (P < 0.002). CONCLUSION: As WJSCs seeded on amniotic membrane could significantly accelerate the healing effect in chronic diabetic wounds, they can be an alternative source in tissue engineering and repair of chronic ulcers.
Authors: O A Krasilnikova; D S Baranovskii; A V Lyundup; P V Shegay; A D Kaprin; I D Klabukov Journal: Stem Cell Rev Rep Date: 2022-04-27 Impact factor: 6.692