Literature DB >> 31127576

Chromatin Immunoprecipitation (ChIP) of Plasmid-Bound Proteins in Xenopus Egg Extracts.

Kelly B Wolfe1, David T Long2.   

Abstract

Xenopus egg extracts provide a cell-free system to analyze various aspects of chromatin biology. Here we describe a modified method of chromatin immunoprecipitation (ChIP) to detect the interaction of proteins with plasmid DNA incubated in extract. The combination of ChIP and Xenopus egg extracts provides a highly versatile and tractable approach to analyze dynamic protein-DNA interactions with great spatial and temporal detail.

Entities:  

Keywords:  Cell-free system; Chromatin immunoprecipitation (ChIP); DNA repair; Nucleoplasmic extract; Xenopus egg extract

Mesh：

Substances：

Year:  2019        PMID： 31127576      PMCID： PMC8091904          DOI： 10.1007/978-1-4939-9500-4_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

1.  Analysis of nuclear reconstitution, nuclear envelope assembly, and nuclear pore assembly using Xenopus in vitro assays.

Authors:  Cyril Bernis; Douglass J Forbes
Journal:  Methods Cell Biol       Date:  2014       Impact factor: 1.441

Review 2.  The current state of chromatin immunoprecipitation.

Authors:  Philippe Collas
Journal:  Mol Biotechnol       Date:  2010-05       Impact factor: 2.695

3.  In vivo interactions of RNA polymerase II with genes of Drosophila melanogaster.

Authors:  D S Gilmour; J T Lis
Journal:  Mol Cell Biol       Date:  1985-08       Impact factor: 4.272

4.  BRCA1 promotes unloading of the CMG helicase from a stalled DNA replication fork.

Authors:  David T Long; Vladimir Joukov; Magda Budzowska; Johannes C Walter
Journal:  Mol Cell       Date:  2014-09-11       Impact factor: 17.970

5.  Xenopus in vitro assays to analyze the function of transmembrane nucleoporins and targeting of inner nuclear membrane proteins.

Authors:  Nathalie Eisenhardt; Allana Schooley; Wolfram Antonin
Journal:  Methods Cell Biol       Date:  2014       Impact factor: 1.441

6.  p97 Promotes a Conserved Mechanism of Helicase Unloading during DNA Cross-Link Repair.

Authors:  George Fullbright; Halley B Rycenga; Jordon D Gruber; David T Long
Journal:  Mol Cell Biol       Date:  2016-11-14       Impact factor: 4.272

7.  Detecting protein-DNA interactions in vivo: distribution of RNA polymerase on specific bacterial genes.

Authors:  D S Gilmour; J T Lis
Journal:  Proc Natl Acad Sci U S A       Date:  1984-07       Impact factor: 11.205

8.  The MCM8-MCM9 complex promotes RAD51 recruitment at DNA damage sites to facilitate homologous recombination.

Authors:  Jonghoon Park; David T Long; Kyung Yong Lee; Tarek Abbas; Etsuko Shibata; Masamitsu Negishi; Yunhai Luo; John C Schimenti; Agnieszka Gambus; Johannes C Walter; Anindya Dutta
Journal:  Mol Cell Biol       Date:  2013-02-11       Impact factor: 4.272

9.  Preparation and use of Xenopus egg extracts to study DNA replication and chromatin associated proteins.

Authors:  Peter J Gillespie; Agnieszka Gambus; J Julian Blow
Journal:  Methods       Date:  2012-04-19       Impact factor: 3.608

10.  Mitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts.

Authors:  David Halpin; Petr Kalab; Jay Wang; Karsten Weis; Rebecca Heald
Journal:  PLoS Biol       Date:  2011-12-27       Impact factor: 8.029
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  2 in total

1.  BRD4 promotes resection and homology-directed repair of DNA double-strand breaks.

Authors:  John K Barrows; Baicheng Lin; Colleen E Quaas; George Fullbright; Elizabeth N Wallace; David T Long
Journal:  Nat Commun       Date:  2022-05-31       Impact factor: 17.694

2.  Regulation of Hepatic Gluconeogenesis by Nuclear Receptor Coactivator 6.

Authors:  Gyun-Sik Oh; Si-Ryong Kim; Eun-Sook Lee; Jin Yoon; Min-Kyung Shin; Hyeon Kyoung Ryu; Dong Seop Kim; Seung-Whan Kim
Journal:  Mol Cells       Date:  2022-04-30       Impact factor: 5.034
  2 in total

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