| Literature DB >> 31127563 |
Stephen J Gardell1,2, Xiaolei Zhang3, Nidhi Kapoor1,2, Christopher Petucci1,4,5, Paul M Coen6.
Abstract
Acute periods of contractile inactivity cause skeletal muscle atrophy along with profound alterations in tissue metabolism. Hind limb unloading via tail suspension is a commonly used rodent model of muscle atrophy. Here, we describe a sample preparation and LC-MS/MS approach for quantifying specific panels of acylcarnitines, amino acids, and organic acids in small (~8 mg) samples of atrophied mouse soleus following a period of hind limb unloading.Entities:
Keywords: Atrophy; Hind limb unloading; Mass spectrometry; Metabolites; Skeletal muscle
Mesh：
Year: 2019 PMID： 31127563 DOI： 10.1007/978-1-4939-9488-5_22
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745