Aim: Nanomaterials and nanomedicinal products tend to interfere with various commonly used assays, including regulatory required endotoxin detection methods for medicines. We developed a method to quantify endotoxin levels that is compatible with nanomaterials and nanomedicinal products. Materials & methods: The method is based on measuring endotoxin indirectly via 3-hydroxylated fatty acids of lipid-A, using Ultra High Performance Liquid Chromatography coupled with mass spectrometry. The outcome was related to results of the commonly used Limulus Amebocyte Lysate method. Results: The ultra high performance liquid chromatography coupled with mass spectrometry method has clear advantages compared with other endotoxin determination assays; particularly the absence of nanospecific interference. Conclusion: The method is sensitive, straightforward and accurate in determining and quantifying endotoxin in nanomedicinal product samples.
Aim: Nanomaterials and nanomedicinal products tend to interfere with various commonly used assays, including regulatory required endotoxin detection methods for medicines. We developed a method to quantify endotoxin levels that is compatible with nanomaterials and nanomedicinal products. Materials & methods: The method is based on measuring endotoxin indirectly via 3-hydroxylated fatty acids of lipid-A, using Ultra High Performance Liquid Chromatography coupled with mass spectrometry. The outcome was related to results of the commonly used Limulus Amebocyte Lysate method. Results: The ultra high performance liquid chromatography coupled with mass spectrometry method has clear advantages compared with other endotoxin determination assays; particularly the absence of nanospecific interference. Conclusion: The method is sensitive, straightforward and accurate in determining and quantifying endotoxin in nanomedicinal product samples.
Authors: Kukka Aimonen; Mira Hartikainen; Monireh Imani; Satu Suhonen; Gerard Vales; Carlos Moreno; Hanna Saarelainen; Kirsi Siivola; Esa Vanhala; Henrik Wolff; Orlando J Rojas; Hannu Norppa; Julia Catalán Journal: Biomacromolecules Date: 2022-06-09 Impact factor: 6.978
Authors: Kukka Aimonen; Monireh Imani; Mira Hartikainen; Satu Suhonen; Esa Vanhala; Carlos Moreno; Orlando J Rojas; Hannu Norppa; Julia Catalán Journal: Part Fibre Toxicol Date: 2022-03-16 Impact factor: 9.400
Authors: Susan Dekkers; James G Wagner; Rob J Vandebriel; Elyse A Eldridge; Selina V Y Tang; Mark R Miller; Isabella Römer; Wim H de Jong; Jack R Harkema; Flemming R Cassee Journal: Part Fibre Toxicol Date: 2019-10-28 Impact factor: 9.400
Authors: Christina Giannakou; Margriet V D Z Park; Irene E M Bosselaers; Wim H de Jong; Jan Willem van der Laan; Henk van Loveren; Rob J Vandebriel; Robert E Geertsma Journal: Wiley Interdiscip Rev Nanomed Nanobiotechnol Date: 2020-04-07
Authors: Teresa Alejo; Laura Uson; Guillermo Landa; Martin Prieto; Cristina Yus Argón; Sara Garcia-Salinas; Ricardo de Miguel; Ana Rodríguez-Largo; Silvia Irusta; Victor Sebastian; Gracia Mendoza; Manuel Arruebo Journal: ACS Appl Mater Interfaces Date: 2021-04-06 Impact factor: 9.229