Small-molecule fluorescent probes for specific detection and imaging of chemical species inside lysosomes.

In the past few years, the preparation of novel small-molecule fluorescent probes for specific detection and imaging of chemical species inside lysosomes has attracted considerable attention because of their wide applications in chemistry, biology, and medical science. This feature article summarizes the recent advances in the design and preparation of small-molecule fluorescent probes for specific detection of chemical species inside lysosomes. In addition, their properties and applications for the detection and imaging of pH, H2O2, HOCl, O2˙-, lipid peroxidation, H2S, HSO3-, thiols, NO, ONOO-, HNO, Zn2+, Cu2+, enzymes, etc. in lysosomes are discussed as well.