Construction of Dopamine-Releasing Gold Surfaces Mimicking Presynaptic Membrane by On-Chip Electrochemistry.

We report a strategy to construct a dopamine-releasing gold surface mimicking a presynaptic membrane on a microfluidic chip to simulate in vivo neural signaling. We constructed dopamine self-assembled monolayers (DA SAMs) by electrochemical deprotection of methyl group-protected DA SAMs on a gold surface. Electrochemically controllable release of DA SAMs can be realized by applying nonhydrolytic negative potential on the gold surface. Our method in constructing DA SAMs avoids the polymerization and protonation of DA molecules which may lead to the failure of the DA SAM formation. By combining microfluidics, we realized spatial and temporal controllable release of DA by electrochemistry from the gold surface. Furthermore, by culturing neurons on the patterned DA SAMs, the interface between the DA SAMs and the neurons could serve as a presynaptic membrane, and the spatiotemporal release of DA could modulate the neuron activity with high precision. Our study holds great promise in the fields of neurobiology research and drug screening.