| Literature DB >> 3111550 |
E Termine, J Léonardi, H Lafont, G Nalbone.
Abstract
Two methods were used to estimate the intracellular phospholipase activity in rat heart: one using exogenous radioactive substrate dispersed as unilamellar vesicles; the other using endogenous membrane hydrolysis and subsequent phospholipid and lysophospholipid separation by high-performance liquid chromatography and quantification by phosphorus determination. We found that the endogenous method provided a higher hydrolysis rate than the exogenous method and that phosphatidyl ethanolamine was a better substrate than phosphatidyl choline.Entities:
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Year: 1987 PMID： 3111550 DOI： 10.1016/0300-9084(87)90049-6
Source DB: PubMed Journal: Biochimie ISSN： 0300-9084 Impact factor: 4.079