Laura de Fátima Souto Maior1,2, Panmella Pereira Maciel3,4, Victor Yuri Nicolau Ferreira3,4, Cíntia de Lima Gouveia Dantas3,4, Jeferson Muniz de Lima3,4, Lúcio Roberto Cançado Castellano3,5, André Ulisses Dantas Batista3,6, Paulo Rogério Ferreti Bonan3,7. 1. School of Dentistry, Federal University of Paraiba, Castelo Branco, Joao Pessoa, Paraiba, Brazil. laurasoutomaior.lsm@gmail.com. 2. Dentistry Post-Graduation Program, Federal University of Paraiba, Campus I / Cidade Universitaria, Joao Pessoa, Paraiba, Brazil. laurasoutomaior.lsm@gmail.com. 3. School of Dentistry, Federal University of Paraiba, Castelo Branco, Joao Pessoa, Paraiba, Brazil. 4. Dentistry Post-Graduation Program, Federal University of Paraiba, Campus I / Cidade Universitaria, Joao Pessoa, Paraiba, Brazil. 5. Dental Graduation Program and Health Technical School, Federal University of Paraiba, Joao Pessoa, Brazil. 6. Dental Graduation Program and Department of Restorative Dentistry, Federal University of Paraiba, Joao Pessoa, Brazil. 7. Dental Graduation Program and Department of Clinic and Social Dentistry, Federal University of Paraiba, Joao Pessoa, Brazil.
Abstract
PURPOSE: This study investigated the anti-Candida activity and the Shore A hardness of a tissue conditioner (Softone™) modified by incorporation of terpinen-4-ol and cinnamaldehyde. MATERIAL AND METHODS: Agar diffusion, microdilution, and mechanism of action methods were performed to determine to evaluate the antifungal activity of phytoconstituents. Then, phytoconstituents in varying concentrations were incorporated into the tissue conditioner. The anti-Candida effect of the modified conditioner was evaluated through agar punch well and biofilm formation methods. Shore A hardness of the experimental liners was evaluated after baseline, 24 h, 48 h, 4 days, and 7 days immersion on artificial saliva. RESULTS: The phytoconstituents incorporated into Softone showed completely inhibited fungal growth in concentrations of 20-40% and did not present significant antifungal activity until their concentrations where higher than 5%. There were differences between non-modified Softone and M5, M10, C10, and T10% (p < 0.05). The groups containing 10-40% of cinnamaldehyde incorporated into Softone were able to completely inhibit the biofilm. Concentrations below 40% of terpinen-4-ol showed unsatisfactory biofilm inhibition. The T40% and C40% groups presented the lowest Shore A hardness values. Hardness values from groups T40% at 7 days (p = 0.476); C40% at 4 days (p = 0.058); and T20% (p = 0.058), C20% (p = 0.205), T30% (p = 0.154), and C30% (p = 0.874) after 48 h did not differ from the control group. CONCLUSIONS: Cinnamaldehyde incorporated into Softone inhibited Candida biofilm formation at concentrations of 10-40%, being more effective than terpinen-4-ol modification despite of halo inhibition observed by both products. CLINICAL RELEVANCE: All modifications showed a very similar pattern of hardness being useful for clinical practice.
PURPOSE: This study investigated the anti-Candida activity and the Shore A hardness of a tissue conditioner (Softone™) modified by incorporation of terpinen-4-ol and cinnamaldehyde. MATERIAL AND METHODS:Agar diffusion, microdilution, and mechanism of action methods were performed to determine to evaluate the antifungal activity of phytoconstituents. Then, phytoconstituents in varying concentrations were incorporated into the tissue conditioner. The anti-Candida effect of the modified conditioner was evaluated through agar punch well and biofilm formation methods. Shore A hardness of the experimental liners was evaluated after baseline, 24 h, 48 h, 4 days, and 7 days immersion on artificial saliva. RESULTS: The phytoconstituents incorporated into Softone showed completely inhibited fungal growth in concentrations of 20-40% and did not present significant antifungal activity until their concentrations where higher than 5%. There were differences between non-modified Softone and M5, M10, C10, and T10% (p < 0.05). The groups containing 10-40% of cinnamaldehyde incorporated into Softone were able to completely inhibit the biofilm. Concentrations below 40% of terpinen-4-ol showed unsatisfactory biofilm inhibition. The T40% and C40% groups presented the lowest Shore A hardness values. Hardness values from groups T40% at 7 days (p = 0.476); C40% at 4 days (p = 0.058); and T20% (p = 0.058), C20% (p = 0.205), T30% (p = 0.154), and C30% (p = 0.874) after 48 h did not differ from the control group. CONCLUSIONS:Cinnamaldehyde incorporated into Softone inhibited Candida biofilm formation at concentrations of 10-40%, being more effective than terpinen-4-ol modification despite of halo inhibition observed by both products. CLINICAL RELEVANCE: All modifications showed a very similar pattern of hardness being useful for clinical practice.
Authors: Maria Clerya Alvino Leite; André Parente de Brito Bezerra; Janiere Pereira de Sousa; Felipe Queiroga Sarmento Guerra; Edeltrudes de Oliveira Lima Journal: Evid Based Complement Alternat Med Date: 2014-08-28 Impact factor: 2.629
Authors: Ramzi A A Mothana; Salah A A Abdo; Sidgi Hasson; Faisal M N Althawab; Sama A Z Alaghbari; Ulrike Lindequist Journal: Evid Based Complement Alternat Med Date: 2008-01-28 Impact factor: 2.629
Authors: Marco Antônio Lavorato de Almeida; André Ulisses Dantas Batista; Maria Rejane Cruz de Araújo; Vanessa Fabiana Dei Santi de Almeida; Paulo Rogério Ferreti Bonan; Danielle Nóbrega Alves; Tereza Karla Vieira Lopes da Costa; Diego Figueiredo Nóbrega; Ricardo Dias de Castro Journal: BMC Oral Health Date: 2020-08-17 Impact factor: 2.757