Emmanouil Chatzipetros1, Zafeiroula Yfanti2, Panos Christopoulos3, Catherine Donta2, Spyros Damaskos2, Evangelos Tsiambas4, Dimitris Tsiourvas5, Eleni-Marina Kalogirou6, Konstantinos I Tosios6, Kostas Tsiklakis2. 1. Department of Oral Diagnosis and Radiology, Faculty of Dentistry, National and Kapodistrian University of Athens, 2 Thivon Str, 11527, Goudi, Athens, Greece. e.chatzipetros@gmail.com. 2. Department of Oral Diagnosis and Radiology, Faculty of Dentistry, National and Kapodistrian University of Athens, 2 Thivon Str, 11527, Goudi, Athens, Greece. 3. Department Oral and Maxillofacial Surgery, Faculty of Dentistry, National and Kapodistrian University of Athens, Athens, Greece. 4. Department of Immunochistochemistry and Molecular Biology, 401 Military Hospital of Athens, Athens, Greece. 5. Institute of Nanoscience and Nanotechnology, National Centre for Scientific Research "Demokritos", Aghia Paraskevi, Attiki, Greece. 6. Department of Oral Medicine and Pathology, Faculty of Dentistry, National and Kapodistrian University of Athens, Athens, Greece.
Abstract
OBJECTIVES: Τhis study aims at determining the ability of cone beam computed tomography (CBCT) to visualize critical-size defects (CSD) created at rat calvaria and filled with 75/25 w/w nano-hydroxyapatite/chitosan (nHAp/CS) scaffolds, prior to their histological investigation. MATERIALS AND METHODS: Thirty adult Sprague Dawley rats, 15 males and 15 females, were used. Two CSD, 5 mm in diameter, were bilaterally trephined in the parietal bone. The right CSD was filled with nHAp/CS scaffold, while the left CSD remained empty, as the control group. Two female rats died post-operatively. Rats were euthanized at 2, 4, and 8 weeks post-surgery. Twenty-eight specimens (15 × 2 × 10 mm) were resected-containing both CSDs-and then scanned using a NewTom VGi CBCT imaging unit (Verona, Italy). The manufacturer's software trace region profile tool (NNT v6.2, Verona, Italy) was used in selected axial slices. The greyscale value (in VGiHU) and the traced/selected region of interest (ROI, in mm2) of those areas were automatically calculated. Subsequently, all specimens were histologically examined. RESULTS: An increased VGiHU (P = 0.000), was observed in the experimental group relative to the control group. The ROI of CSD (in mm2) was significantly reduced (P = 0.001) from the fourth to the eighth week in both groups. No statistically significant difference between male and female rats (P = 0.188) was observed with respect to VGiHU. CONCLUSIONS: The nHAp/CS scaffolds are easily visualized using a particular high-resolution CBCT device. CLINICAL RELEVANCE: Both the CBCT measurements and also the histological results suggest that the nHAp/CS scaffold presence contributes to new bone formation in rat calvarial CSD.
OBJECTIVES: Τhis study aims at determining the ability of cone beam computed tomography (CBCT) to visualize critical-size defects (CSD) created at rat calvaria and filled with 75/25 w/w nano-hydroxyapatite/chitosan (nHAp/CS) scaffolds, prior to their histological investigation. MATERIALS AND METHODS: Thirty adult Sprague Dawley rats, 15 males and 15 females, were used. Two CSD, 5 mm in diameter, were bilaterally trephined in the parietal bone. The right CSD was filled with nHAp/CS scaffold, while the left CSD remained empty, as the control group. Two female rats died post-operatively. Rats were euthanized at 2, 4, and 8 weeks post-surgery. Twenty-eight specimens (15 × 2 × 10 mm) were resected-containing both CSDs-and then scanned using a NewTom VGi CBCT imaging unit (Verona, Italy). The manufacturer's software trace region profile tool (NNT v6.2, Verona, Italy) was used in selected axial slices. The greyscale value (in VGiHU) and the traced/selected region of interest (ROI, in mm2) of those areas were automatically calculated. Subsequently, all specimens were histologically examined. RESULTS: An increased VGiHU (P = 0.000), was observed in the experimental group relative to the control group. The ROI of CSD (in mm2) was significantly reduced (P = 0.001) from the fourth to the eighth week in both groups. No statistically significant difference between male and female rats (P = 0.188) was observed with respect to VGiHU. CONCLUSIONS: The nHAp/CS scaffolds are easily visualized using a particular high-resolution CBCT device. CLINICAL RELEVANCE: Both the CBCT measurements and also the histological results suggest that the nHAp/CS scaffold presence contributes to new bone formation in rat calvarial CSD.
Authors: Tabata P Sato; Bruno V M Rodrigues; Daphne C R Mello; Eliseu A Münchow; Juliana S Ribeiro; João Paulo B Machado; Luana M R Vasconcellos; Anderson O Lobo; Marco C Bottino; Alexandre L S Borges Journal: Clin Oral Investig Date: 2020-10-13 Impact factor: 3.573
Authors: Carlos Humberto Valencia-Llano; Diego López-Tenorio; Marcela Saavedra; Paula A Zapata; Carlos David Grande-Tovar Journal: Molecules Date: 2022-09-06 Impact factor: 4.927