MiR-449b-5p regulates cell proliferation, migration and radioresistance in cervical cancer by interacting with the transcription suppressor FOXP1.

MicroRNAs (miRNAs) have been acknowledged as crucial regulators for the malignant progression of human cancers. However, the molecular mechanism associated with the malignant progression of cervical cancer (CC) is still largely unmarked. The focus of this study is to explore the potential functional mechanism of miR-449b-5p in CC. Using qRT-PCR analysis, we detected a relative lower expression level of miR-449b-5p in CC tissues and cell lines by comparing with the normal tissues and cells. Low level of miR-449b-5p in CC cell lines was further demonstrated by northern blot. Subsequently, downregulation of miR-449b-5p was closely correlated with the low overall survival rate of patients with cervical cancer. Gain-of-function assays demonstrated that upregulation of miR-449b-5p had an inhibitory effect on cell proliferation, migration and invasion. Moreover, FOXP1 was found to be a transcription suppressor and downstream target of miR-449b-5p. Rescue assays and in vivo experiment were applied to demonstrate the biological function of miR-449b-5p-FOXP1 feedback loop. In summary, miR-449b-5p interacted with FOXP1 to regulate cell proliferation, migration, invasion and radiosensitivity in CC.