| Literature DB >> 3109866 |
M Nishizawa, F Ozawa, F Hishinuma.
Abstract
An amylase gene of Bacillus circulans was cloned in B. subtilis and its nucleotide sequence was determined. The putative proamylase consists of 528 amino acids, which correspond to a molecular weight of 58,776. Homologous regions with other amylases of Bacillus species were found. A sigma 55-type promoter is located at about 250 bp upstream from the starting codon. This promoter was also functional in Escherichia coli, and able to express beta-galactosidase activity.Entities:
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Year: 1987 PMID: 3109866 DOI: 10.1089/dna.1987.6.255
Source DB: PubMed Journal: DNA ISSN: 0198-0238