| Literature DB >> 31097705 |
Vasilios Tsarouhas1,2, Dan Liu1, Georgia Tsikala1, Alina Fedoseienko3, Kai Zinn4, Ryo Matsuda1, Daniel D Billadeau3, Christos Samakovlis5,6,7.
Abstract
Filamentous actin (F-actin) networks facilitate key processes like cell shape control, division, polarization and motility. The dynamic coordination of F-actin networks and its impact on cellular activities are poorly understood. We report an antagonistic relationship between endosomal F-actin assembly and cortical actin bundle integrity during Drosophila airway maturation. Double mutants lacking receptor tyrosine phosphatases (PTP) Ptp10D and Ptp4E, clear luminal proteins and disassemble apical actin bundles prematurely. These defects are counterbalanced by reduction of endosomal trafficking and by mutations affecting the tyrosine kinase Btk29A, and the actin nucleation factor WASH. Btk29A forms protein complexes with Ptp10D and WASH, and Btk29A phosphorylates WASH. This phosphorylation activates endosomal WASH function in flies and mice. In contrast, a phospho-mimetic WASH variant induces endosomal actin accumulation, premature luminal endocytosis and cortical F-actin disassembly. We conclude that PTPs and Btk29A regulate WASH activity to balance the endosomal and cortical F-actin networks during epithelial tube maturation.Entities:
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Year: 2019 PMID： 31097705 PMCID： PMC6522504 DOI： 10.1038/s41467-019-10229-6
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919