Literature DB >> 3109401

Studies on the biotin-binding site of avidin. Lysine residues involved in the active site.

G Gitlin, E A Bayer, M Wilchek.   

Abstract

Egg-white avidin was treated with 1-fluoro-2,4-dinitrobenzene. Modification of an average of one lysine residue per avidin subunit caused the complete loss of biotin binding. Tryptic peptides obtained from the 2,4-dinitrophenylated avidin were fractionated by reversed-phase h.p.l.c. Three peptides contained the 2,4-dinitrophenyl group. Amino acid analysis revealed that lysine residues 45, 94 and 111 are modified and probably comprise part of the biotin-binding site.

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Year:  1987        PMID： 3109401      PMCID： PMC1147797          DOI： 10.1042/bj2420923

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

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Journal:  Biochem J       Date:  1963-12       Impact factor: 3.857

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3.  Spectroscopic evidence for the participation of tryptophan residues in the binding of biotin by avidin.

Authors:  N M GREEN
Journal:  Biochim Biophys Acta       Date:  1962-05-07

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Journal:  Arch Biochem Biophys       Date:  1952-07       Impact factor: 4.013

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Journal:  J Biol Chem       Date:  1971-02-10       Impact factor: 5.157

7.  Molecular cloning and nucleotide sequence of the streptavidin gene.

Authors:  C E Argaraña; I D Kuntz; S Birken; R Axel; C R Cantor
Journal:  Nucleic Acids Res       Date:  1986-02-25       Impact factor: 16.971

Review 8.  The occurrence and production of avidin: a new conception of the high-affinity biotin-binding protein.

Authors:  H A Elo; J Korpela
Journal:  Comp Biochem Physiol B       Date:  1984

9.  The use of the avidin-biotin complex as a tool in molecular biology.

Authors:  E A Bayer; M Wilchek
Journal:  Methods Biochem Anal       Date:  1980

10.  A sensitive enzyme assay for biotin, avidin, and streptavidin.

Authors:  E A Bayer; H Ben-Hur; M Wilchek
Journal:  Anal Biochem       Date:  1986-04       Impact factor: 3.365
  10 in total
  13 in total

1.  A microfilament formation inhibitor, cytochalasin strongly enhances the low-affinity Fc epsilon receptor II (CD23) expression on the human monocyte-like cell line, U937.

Authors:  N Ikewaki; H Tamauchi; A Yamada; M Aoki; R Yamamoto; A Sawada; H Inoko
Journal:  J Clin Immunol       Date:  2000-11       Impact factor: 8.317

2.  A unique monoclonal antibody mNI-11 rapidly enhances spread formation in human umbilical vein endothelial cells.

Authors:  N Ikewaki; H Tamauchi; A Yamada; N Mori; H Yamao; H Inoue; H Inoko
Journal:  J Clin Immunol       Date:  2000-07       Impact factor: 8.317

3.  Phylogenetic survey of soluble saxitoxin-binding activity in pursuit of the function and molecular evolution of saxiphilin, a relative of transferrin.

Authors:  L E Llewellyn; P M Bell; E G Moczydlowski
Journal:  Proc Biol Sci       Date:  1997-06-22       Impact factor: 5.349

Review 4.  Chemical probes for analysis of carbonylated proteins: a review.

Authors:  Liang-Jun Yan; Michael J Forster
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2010-08-07       Impact factor: 3.205

5.  Postsecretory modifications of streptavidin.

Authors:  E A Bayer; H Ben-Hur; Y Hiller; M Wilchek
Journal:  Biochem J       Date:  1989-04-15       Impact factor: 3.857

6.  Studies on the biotin-binding sites of avidin and streptavidin. A chemically induced dynamic nuclear polarization investigation of the status of tyrosine residues.

Authors:  G Gitlin; I Khait; E A Bayer; M Wilchek; K A Muszkat
Journal:  Biochem J       Date:  1989-04-15       Impact factor: 3.857

7.  Pretargeting of necrotic tumors with biotinylated hypericin using 123I-labeled avidin: evaluation of a two-step strategy.

Authors:  Thierry Marysael; Matthias Bauwens; Yicheng Ni; Guy Bormans; Jef Rozenski; Peter de Witte
Journal:  Invest New Drugs       Date:  2011-12-21       Impact factor: 3.850

8.  Studies on the biotin-binding site of avidin. Tryptophan residues involved in the active site.

Authors:  G Gitlin; E A Bayer; M Wilchek
Journal:  Biochem J       Date:  1988-02-15       Impact factor: 3.857

9.  Studies on the biotin-binding sites of avidin and streptavidin. Tyrosine residues are involved in the binding site.

Authors:  G Gitlin; E A Bayer; M Wilchek
Journal:  Biochem J       Date:  1990-07-15       Impact factor: 3.857

10.  Versatile strategy for the synthesis of biotin-labelled glycans, their immobilization to establish a bioactive surface and interaction studies with a lectin on a biochip.

Authors:  F Javier Muñoz; Angel Rumbero; José V Sinisterra; J Ignacio Santos; Sabine André; Hans-J Gabius; Jesús Jiménez-Barbero; María J Hernáiz
Journal:  Glycoconj J       Date:  2008-03-18       Impact factor: 2.916
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