J Yu1,2, Y Zhao3, C Liu1, B Hu4, M Zhao1, Y Ma1, J Jiang1. 1. Medical College of China, Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Three Gorges University, Yichang, 443002, Hubei Province, China. 2. Department of Ultrasonography, Hubei Cancer Hospital, Wuhan, 430079, Hubei Province, China. 3. Medical College of China, Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Three Gorges University, Yichang, 443002, Hubei Province, China. zhaoyun@ctgu.edu.cn. 4. Department of Ultrasonography, The Second Clinical Medical College of China, Three Gorges University, Yichang, 443002, Hubei Province, China.
Abstract
PURPOSE: Improved therapeutic options for cervical cancer are needed. The purpose of this study was to evaluate the synergetic, inhibitory effects of ultrasound-mediated paclitaxel (PTX)- and miR-34a-loaded microbubbles (MBs) on cervical cancer. METHODS: U14 cervical cancer cells and xenograft mouse tumors were treated with PTX-miR-34a-MBs. RESULTS: Levels of miR-34a increased in vitro and vivo after treatment with ultrasound-mediated PTX-miR-34a-MBs. Furthermore, this treatment decreased the proliferation of cervical cancer cells, microvessel density, and the expression of Bcl-2 and CDK6, both in vitro and in vivo. Furthermore, Bax expression was increased in the in vivo model. And, tumor volume and weight were significantly reduced by 78.57% and 87.97%, respectively (P < 0.01). CONCLUSIONS: These results indicate that ultrasound-mediated PTX-miR-34a-MBs synergistically inhibit the growth of cervical cancer via the upregulation of miR-34a and downregulation of Bcl-2 and CDK6. Thus, PTX-miR-34a-MBs in combination with ultrasound microbubbles are a promising anticancer delivery strategy for treating cervical cancer.
PURPOSE: Improved therapeutic options for cervical cancer are needed. The purpose of this study was to evaluate the synergetic, inhibitory effects of ultrasound-mediated paclitaxel (PTX)- and miR-34a-loaded microbubbles (MBs) on cervical cancer. METHODS: U14 cervical cancer cells and xenograft mousetumors were treated with PTX-miR-34a-MBs. RESULTS: Levels of miR-34a increased in vitro and vivo after treatment with ultrasound-mediated PTX-miR-34a-MBs. Furthermore, this treatment decreased the proliferation of cervical cancer cells, microvessel density, and the expression of Bcl-2 and CDK6, both in vitro and in vivo. Furthermore, Bax expression was increased in the in vivo model. And, tumor volume and weight were significantly reduced by 78.57% and 87.97%, respectively (P < 0.01). CONCLUSIONS: These results indicate that ultrasound-mediated PTX-miR-34a-MBs synergistically inhibit the growth of cervical cancer via the upregulation of miR-34a and downregulation of Bcl-2 and CDK6. Thus, PTX-miR-34a-MBs in combination with ultrasound microbubbles are a promising anticancer delivery strategy for treating cervical cancer.
Authors: K Iwanaga; K Tominaga; K Yamamoto; M Habu; H Maeda; S Akifusa; T Tsujisawa; T Okinaga; J Fukuda; T Nishihara Journal: Cancer Gene Ther Date: 2007-02-02 Impact factor: 5.987
Authors: Lin He; Xingyue He; Lee P Lim; Elisa de Stanchina; Zhenyu Xuan; Yu Liang; Wen Xue; Lars Zender; Jill Magnus; Dana Ridzon; Aimee L Jackson; Peter S Linsley; Caifu Chen; Scott W Lowe; Michele A Cleary; Gregory J Hannon Journal: Nature Date: 2007-06-06 Impact factor: 49.962