A novel immobilized-enzyme system utilizing microcapsules.

A novel immobilized-enzyme system that has glucoamylase on the surface of, and glucose oxidase within, polyurea microcapsules was developed. This system was found to carry out its sequential enzymatic reaction effectively. It was also demonstrated that the use of microcapsules was useful for this immobilized-enzyme system since a large amount of the second enzyme, glucose oxidase, was required for the sequential reaction to proceed efficiently. In addition, this system was found helpful for making the size of reaction batches smaller.