Katarzyna Nowinska1, Urszula Ciesielska2, Aleksandra Piotrowska2, Karolina Jablonska2, Aleksandra Partynska2, Maria Paprocka3, Tomasz Zatonski4, Marzena Podhorska-Okolow5, Piotr Dziegiel2,6. 1. Department of Human Morphology and Embryology, Division of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland katarzyna.nowinska@umed.wroc.pl. 2. Department of Human Morphology and Embryology, Division of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland. 3. Laboratory of Cellular Interactions, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland. 4. Department of Otolaryngology, Head & Neck Surgery, Wroclaw Medical University, Wroclaw, Poland. 5. Division of Ultrastructure Research, Wroclaw Medical University, Wroclaw, Poland. 6. Department of Physiotherapy, Wroclaw University School of Physical Education, Wroclaw, Poland.
Abstract
BACKGROUND/AIM: The minichromosome maintenance proteins (MCMs) may be potential biomarkers of cancer cell proliferation. They are essential to initiate DNA replication. The aim of the study was to investigate the level of MCM5 expression in benign lesions (BLs) and laryngeal squamous cell cancer (LSCC). MATERIALS AND METHODS: Immunohistochemical (IHC) analysis was carried out on 83 LSCCs and 10 BLs. Western-blot, immunofluorescence analysis (IF) and real-time PCR (RT-PCR) were performed using HEp-2 cancer cells and HaCaT keratinocytes. RESULTS: The expression of MCM5 was higher in LSCC than in the BLs (p<0.0001) and was higher in subsequent malignancies of LSCC. Positive correlations were demonstrated between the expression levels of MCM5 and the Ki-67 antigen. In vitro studies have confirmed that the expression of MCM5 is elevated in cancer cells. CONCLUSION: MCM5 protein may be used as a potential marker of cancer cell proliferation in LSCC. Copyright
BACKGROUND/AIM: The minichromosome maintenance proteins (MCMs) may be potential biomarkers of cancer cell proliferation. They are essential to initiate DNA replication. The aim of the study was to investigate the level of MCM5 expression in benign lesions (BLs) and laryngeal squamous cell cancer (LSCC). MATERIALS AND METHODS: Immunohistochemical (IHC) analysis was carried out on 83 LSCCs and 10 BLs. Western-blot, immunofluorescence analysis (IF) and real-time PCR (RT-PCR) were performed using HEp-2 cancer cells and HaCaT keratinocytes. RESULTS: The expression of MCM5 was higher in LSCC than in the BLs (p<0.0001) and was higher in subsequent malignancies of LSCC. Positive correlations were demonstrated between the expression levels of MCM5 and the Ki-67 antigen. In vitro studies have confirmed that the expression of MCM5 is elevated in cancer cells. CONCLUSION:MCM5 protein may be used as a potential marker of cancer cell proliferation in LSCC. Copyright
Authors: Urszula Ciesielska; Aleksandra Piotrowska; Christopher Kobierzycki; Wojciech Pastuszewski; Marzenna Podhorska-Okolow; Piotr Dziegiel; Katarzyna Nowinska Journal: In Vivo Date: 2020 Nov-Dec Impact factor: 2.155