Dedy Hb Wicaksono1, Intan N Syazwani2, Nalin Ratnarathorn3, Sahba Sadir2, Shafinaz Shahir2, Leela Ruckthong3, Wijitar Dungchai3, Brian Yuliarto4,5, Hermawan K Dipojono4,5. 1. Department of Biomedical Engineering, Faculty of Life Science & Technology, Swiss German University (SGU), The Prominence Tower, Jalan Jalur Sutera Barat no. 15, Alam Sutera, Tangerang, 15143 Banten, Indonesia. 2. Faculty of Biosciences & Medical Engineering, Universiti Teknologi Malaysia, UTM, Skudai 81310, Johor, Malaysia. 3. Organic Synthesis, Electrochemistry & Natural Product Research Unit, Department of Chemistry, Faculty of Science, King Mongkut's University of Technology Thonburi (KMUTT), 126 Prachautid Road, Thungkru, Bangkok 10140, Thailand. 4. Department of Engineering Physics, Institut Teknologi Bandung (ITB), Bandung 40132, Indonesia. 5. Research Center for Nanosciences & Nanotechnology, Institut Teknologi Bandung (ITB), Bandung 40132, Indonesia.
Abstract
Aim: Time-based microfluidic absorption sampling was proposed using cotton fiber-based device made in swab stick. The assay was optimized and compared with conventional pipetted drop sampling using the same device. Materials & methods: Reagents were integrated into cotton fiber device for assessing concentration of analytes by the colorimetric detection method through time-based absorption sampling microfluidic system. All assay parameters were first optimized using conventional pipette-based drop sampling. Results: The color intensity is linear in the relevant concentration range of the analytes. The LOD are 0.189 mM for glucose and 6.56 μM for nitrite, respectively. These values are better than conventional drop sampling. The fiber-containing swab itself functions as sampling, assay and calibration device. Conclusion: Microfluidic cotton fiber-based assay device was fabricated and can determine analyte concentration in artificial salivary samples, colorimetrically, by time-based absorption sampling without the need of complex equipments.
Aim: Time-based microfluidic absorption sampling was proposed using cotton fiber-based device made in swab stick. The assay was optimized and compared with conventional pipetted drop sampling using the same device. Materials & methods: Reagents were integrated into cotton fiber device for assessing concentration of analytes by the colorimetric detection method through time-based absorption sampling microfluidic system. All assay parameters were first optimized using conventional pipette-based drop sampling. Results: The color intensity is linear in the relevant concentration range of the analytes. The LOD are 0.189 mM for glucose and 6.56 μM for nitrite, respectively. These values are better than conventional drop sampling. The fiber-containing swab itself functions as sampling, assay and calibration device. Conclusion: Microfluidic cotton fiber-based assay device was fabricated and can determine analyte concentration in artificial salivary samples, colorimetrically, by time-based absorption sampling without the need of complex equipments.